Czech J. Anim. Sci., 2018, 63(4):127-135 | DOI: 10.17221/79/2017-CJAS

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motilityOriginal Paper

Tomáı Slanina*,1, Michal Miıkeje2, Filip Tirpák1, Martyna B³aszczyk3, Robert Stawarz3, Peter Massányi1,3
1 Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovakia
2 AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Nitra, Slovakia
3 Department of Zoology of Vertebrates and Human Biology, Institute of Biology, Pedagogical University of Krakow, Krakow, Poland

The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine - from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87-9.90%) if compared to 5°C (2.12-4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

Keywords: amino acid; liquid storage; in vitro; CASA system; membrane integrity; fowl

Published: April 30, 2018  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Slanina T, Miıkeje M, Tirpák F, B³aszczyk M, Stawarz R, Massányi P. Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility. Czech J. Anim. Sci. 2018;63(4):127-135. doi: 10.17221/79/2017-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Alvarez J.G., Storey B.T. (1983): Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biology of Reproduction, 29, 548-555. Go to original source... Go to PubMed...
    2. Blaszczyk M., Slanina T., Massanyi P., Stawarz R. (2013): Semen quality assessment of New Zealand white rabbit bucks. Journal of Microbiology, Biotechnology and Food Sciences, 2, 2168-2179.
    3. Bucak M., Tekin N. (2007): Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research, 73, 103-108. Go to original source...
    4. Bucak M.N., Atessahin A., Varisli O., Yuce A., Tekin N., Akcay A. (2007): The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen. Microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology, 67, 1060-1067. Go to original source... Go to PubMed...
    5. Chhillar S., Snigh V.K., Kumar R., Atreja S.K. (2012): Effects of taurine or trehalose supplementation on functional competence of cryopreserved Karan Fries semen. Animal Reproduction Sciences, 135, 1-7. Go to original source... Go to PubMed...
    6. Dawson R., Wallace D.R. (1992): Taurine content in tissues from aged Fischer 344 rats. Age, 15, 73-81. Go to original source...
    7. Froman D.P., McLean D.J. (1996): Objective measurement of sperm motility based upon sperm penetration of Accudenz®. Poultry Science, 75, 776-784. Go to original source... Go to PubMed...
    8. Guerin P., Guillaud J., Menezo Y. (1995): Hypotaurine in spermatozoa and genital secretions and its production by oviduct epithelial cells in vitro. Human Reproduction, 10, 866-872. Go to original source... Go to PubMed...
    9. Gutierrez-Perez O., Juarez-Mosqueda M.L., Carvajal S.U., Ortega M.E.T. (2009): Boar spermatozoa cryopreservation in low glycerol/trehalose enriched freezing media improves cellular integrity. Cryobiology, 58, 287-292. Go to original source... Go to PubMed...
    10. Hu J.H., Li Q.W., Jiang Z.L., Yang H., Zhang S.S., Zhao H.W. (2009): The cryoprotective effect of trehalose supplementation on boar spermatozoa quality. Reproduction in Domestic Animals, 44, 571-575. Go to original source... Go to PubMed...
    11. King L.M., Holsberger D.R., Donoghue A.M. (2000): Correlation of CASA velocity and linearity parameters with sperm mobility phenotype in turkeys. Journal of Andrology, 21, 65-71. Go to PubMed...
    12. Krockova J., Massanyi P., Toman R., Danko J., Roychoudhury S. (2012): In vivo and in vitro effect of bendiocarb on rabbit testicular structure and spermatozoa motility. Journal of Environmental Science and Health. Part A, Toxic/Hazardous Substances and Environmental Engineering, 47, 1301-1311. Go to original source... Go to PubMed...
    13. Kumar R., Atreja S.K. (2012): Effect of incorporation of additives in tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm tyrosine phosphorylation during cryopreservation. Reproduction in Domestic Animals, 47, 485-490. Go to original source... Go to PubMed...
    14. Li J.H., Ling Y.Q., Fan J.J., Zhang X.P., Cui S. (2006): Expression of cysteine sulfinate decarboxylase (CSD) in male reproductive organs of mice. Histochemistry and Cell Biology, 125, 607-613. Go to original source... Go to PubMed...
    15. Lobo M.V.T., Alonso F.J.M., Rio R.M. (2000): Immunohistochemical localization of taurine in the male reproductive organs of the rat. Journal of Histochemistry and Cytochemistry, 48, 313-320. Go to original source... Go to PubMed...
    16. Martinez-Paramoa S., Diogoa P., Dinisa M.T., Soaresb F., Sarasquetec C., Cabritac E. (2013): Effect of two sulfur-containing amino acids, taurine and hypotaurine in European sea bass (Dicentrarchuslabrax) sperm cryopreservation. Cryobiology, 66, 333-338. Go to original source... Go to PubMed...
    17. Martins-Bessa A., Rocha A., Mayenco-Aguirre A. (2009): Effects of taurine and hypotaurine supplementation and ionophore concentrations on post-thaw acrosome reaction of dog spermatozoa. Theriogenology, 71, 248-253. Go to original source... Go to PubMed...
    18. Massanyi P., Weis J., Lukac N., Trandzik J., Bystricka J. (2008): Cadmium, zinc, copper, sodium and potassium concentrations in rooster and turkey semen and their correlation. Journal of Environmental Science and Health, Part A, Toxic/Hazardous Substances and Environmental Engineering, 43, 563-565. Go to original source... Go to PubMed...
    19. Partyka A., Rodak O., Bajzert J., Kochan J., Nizanski W. (2017): The effect of l-carnitine, hypotaurine, and taurine supplementation on the quality of cryopreserved chicken semen. BioMed Research International, 2017, Article ID 7279341. Go to original source...
    20. Perumal P., Kezhavituo V., Rajkhowa C. (2013): Effect of addition of taurine on the liquid storage (5°C) of mithun (Bos frontalis) semen. Veterinary Medicine International, 2013, 1-7. Go to original source... Go to PubMed...
    21. Reddy N.S.S., Mohanarao G.J., Atreja S.K. (2010): Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus Bubalis) sperm quality following cryopreservation. Animal Reproduction Science, 119, 183-190. Go to original source... Go to PubMed...
    22. Sanchez-Partida L.G., Setchell B.P., Maxwell W.M.C. (1997): Epididymal compounds and antioxidants in diluents for the frozen storage of ram spermatozoa. Reproduction, Fertility, and Development, 9, 689-696. Go to original source... Go to PubMed...
    23. Sariozkan S., Bucak M.N., Tuncer P.B., Ulutas P.A., Bilgen A. (2009): The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology, 58, 134-138. Go to original source... Go to PubMed...
    24. Slanina T., Miskeje M., Knizat L., Mirda J., Massanyi P. (2012): The effect of different concentration of trehalose on turkey spermatozoa motility in vitro. Journal of Microbiology, Biotechnology and Food Sciences, 4, 573-582.
    25. Slanina T., Miskeje M., Petrovicova I., Lukac N., Massanyi P. (2015a): Changes in turkey spermatozoa motility parameters after addition of copper sulphate in vitro. Journal of Microbiology, Biotechnology and Food Sciences, 4, 98-100. Go to original source...
    26. Slanina T., Petrovicova L., Miskeje M., Knizat L., Mirda J., Lukac N., Trandzik J., Petrovicova I., Massanyi P. (2015b): The effect of diluent, temperature and age on turkey spermatozoa motility in vitro. Journal of Applied Animal Research, 43, 131-136. Go to original source...
    27. Slanina T., Kovacova R., Lukac N., Massanyi P. (2016): Effect of tilmicosin on the rabbit spermatozoa motility and viability. Journal of Microbiology, Biotechnology and Food Sciences, 5, 53-56. Go to original source...
    28. Witte T.S., Schafer-Somi S. (2007): Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa. Animal Reproduction Science, 102, 181-193. Go to original source... Go to PubMed...
    29. Yang J., Wu G., Feng Y., Lv Q., Lin S., Hu J. (2010a): Effects of taurine on male reproduction in rats of different ages. Journal of Biomedical Science, 17 (Suppl. 1): S9. Go to original source... Go to PubMed...
    30. Yang J.C., Wu G.F., Feng Y., Sun C.M., Lin S.M., Hu J.M. (2010b): CSD mRNA expression in rat testis and the effect of taurine on testosterone secretion. Amino Acids, 39, 155-160. Go to original source... Go to PubMed...
    31. Yun J.I., Gong S.P., Song Y.H., Lee S.T. (2013): Effects of combined antioxidant supplementation on human sperm motility and morphology during sperm manipulation in vitro. Fertility and Sterility, 100, 373-378. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content