Czech J. Anim. Sci., 2016, 61(7):326-332 | DOI: 10.17221/69/2015-CJAS

Alteration of parameters of energy metabolism and ATPase enzymatic system in juvenile common carp (Cyprinus carpio) chronically exposed to tributyltinOriginal Paper

Z.-H. Li^1,2, L.-Q. Zhong¹, W.-N. Mu^1,3, Y.-H. Wu^1,4

¹ Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, P.R. China

² South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic

³ College of Fisheries, Huazhong Agricultural University, Wuhan, P.R. China

⁴ Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing, P.R. China

The effect of long-term exposure to tributyltin (TBT) on energy metabolism and adenosine triphosphatase (ATPase) enzymatic system in freshwater teleost was investigated. The impact on the parameters of energy metabolism (the content of glucose (Glu) and lactate (LA), and the activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase(LDH)) in the muscle tissue, as well as on the ATP enzymatic system (ATP content, Total-ATPase, Na⁺-K⁺-ATPase, and Ca²⁺-Mg²⁺-ATPase) in the gill tissue of common carp was evaluated. Fish were exposed to sublethal concentrations of TBT (75, 0.75, and 7.5 µg/l) for 60 days. Based on the results, long-term exposure to TBT could lead to obvious ATP enzymatic system responses, including the decreased ATP content and Na⁺-K⁺-ATPase activity, and the increased activities of Total-ATPase and Ca²⁺-Mg²⁺-ATPase. Moreover, the parameters of energy metabolism in muscle were also regulated, such as induced indices of the levels of Glu and LA, and the activities of HK and PK, and inhibited indices of LDH activity. Shortly, the measured physiological responses in fish could provide useful information to better understand the mechanisms of TBT-induced bio-toxicity, and could be used as potential biomarkers for monitoring the TBT pollution in the field.

Keywords: organotin; fish; physiological indices; chronic toxicity; biomarkers

Published: July 31, 2016  Show citation