Czech J. Anim. Sci., 2016, 61(4):186-193 | DOI: 10.17221/8850-CJAS

Effects of dietary conjugated linoleic acid on the duodenal mucosal immunity response and redox status of broiler chicks infected with Eimeria aceruvlina Original Paper

Y.X. Liu1, Y.L. Liu1, J.P. Yang2, W.T. Li2
1 College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, P.R. China
2 Henan Engineering Technology Research Center of Animal Nutrition and Feed, Zhengzhou, P.R. China

The present experiment investigated the effects of conjugated linoleic acid (CLA) on the growth performance, oocyst shedding, duodenal mucosal immune response, and redox status of broiler chicks infected with Eimeria acervulina. Totally 144 birds were fed either the basal diet or 1.5% CLA diet for 21 days, and then half of them from each dietary treatment were inoculated with 10 000 Eimeria acervulina sporulated oocysts. Compared with the basal diet, the 1.5% CLA diet partially ameliorated the growth inhibition (P < 0.01) and decreased the oocyst shedding of broiler chicks at ca. 6-9 days post-inoculation (P < 0.01). Additionally the dietary CLA significantly increased the percentage of CD8+ T lymphocytes in the duodenal epithelium (P < 0.05), and showed no obvious effect on the interferon γ expression in duodenal mucosa (P > 0.05). The dietary CLA also notably suppressed the increase of malondialdehyde (P < 0.05) and the decrease of glutathione concentration (P < 0.01) in duodenal mucosa of the infected birds, without changing the activities of total superoxide dismutase and catalase (P > 0.05). These results demonstrate the protective effect of dietary CLA on the broiler chicks infected with Eimeria acervulina, and anticoccidial effects of dietary CLA are most likely related to the increased proportions of CD8+ T lymphocytes in duodenal epithelium and the inhibition on the decrease of duodenal mucosal glutathione after Eimeria acervulina infection.

Keywords: CLA; intestinal mucosal immunity; oxidative stress; broiler chick; coccidiosis

Published: April 30, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Liu YX, Liu YL, Yang JP, Li WT. Effects of dietary conjugated linoleic acid on the duodenal mucosal immunity response and redox status of broiler chicks infected with Eimeria aceruvlina . Czech J. Anim. Sci. 2016;61(4):186-193. doi: 10.17221/8850-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bassaganya-Riera J., Hontecillas-Magarzo R., Bregendahl K., Wannemuehler M.J., Zimmerman D.R. (2001): Effects of dietary conjugated linoleic acid in nursery pigs of dirty and clean environments on growth, empty body composition, and immune competence. Journal of Animal Science, 79, 714-721. Go to original source... Go to PubMed...
    2. Bassaganya-Riera J., Hontecillas R., Zimmerman D.R., Wannemuehler M.J. (2002): Long-term influence of lipid nutrition on the induction of CD8+ responses to viral or bacterial antigens. Vaccine, 20, 1435-1444. Go to original source... Go to PubMed...
    3. Bassaganya-Riera J., Pogranichniy R.M., Jobgen S.C., Halbur P.G., Yoon K.J., O'Shea M., Mohede I., Hontecillas R. (2003): Conjugated linoleic acid ameliorates viral infectivity in a pig model of virally induced immunosuppression. Journal of Nutrition, 133, 3204-3214. Go to original source... Go to PubMed...
    4. Bergamo P., Gogliettino M., Palmieri G., Cocca E., Maurano F., Stefanile R., Balestrieri M., Mazzarella G., David C., Rossi M. (2011): Conjugated linoleic acid protects against gliadin-induced depletion of intestinal defenses. Molecular Nutrition and Food Research, 55, 248-256. Go to original source... Go to PubMed...
    5. Bessay M., Le Vern Y., Kerboeuf D., Yvore P., Quere P. (1996): Changes in intestinal intra-epithelial and systemic T-cell subpopulations after an Eimeria infection in chickens: comparative study between E. acervulina and E. tenella. Veterinary Research, 27, 503-514.
    6. Beutler E. (1975): Disorders in glutathione metabolism. Life Sciences, 16, 1499-1505. Go to original source... Go to PubMed...
    7. Cohen G., Dembiec D., Marcus J. (1970): Measurement of catalase activity in tissue extracts. Analytical Biochemistry, 34, 30-38. Go to original source... Go to PubMed...
    8. Evans N.P., Misyak S.A., Schmelz E.M., Guri A.J., Hontecillas R., Bassaganya-Riera J. (2010): Conjugated linoleic acid ameliorates inflammation-induced colorectal cancer in mice through activation of PPARγ. Journal of Nutrition, 140, 515-521. Go to original source... Go to PubMed...
    9. Georgieva N.V., Koinarski V., Gadjeva V. (2006): Antioxidant status during the course of Eimeria tenella infection in broiler chickens. Veterinary Journal, 172, 488-492. Go to original source... Go to PubMed...
    10. Hong Y.H., Lillehoj H.S., Park D.W., Lee S.H., Han J.Y., Park M.S., Kim J.K. (2008): Cloning and functional characterization of chicken interleukin D. Veterinary Immunology and Immunopathology, 126, 1-8. Go to original source... Go to PubMed...
    11. Hontecillas R., Wannemeulher M.J., Zimmerman D.R., Hutto D.L., Wilson J.H., Ahn D.U., Bassaganya-Riera J. (2002): Nutritional regulation of porcine bacterialinduced colitis by conjugated linoleic acid. Journal of Nutrition, 132, 2019-2027. Go to original source... Go to PubMed...
    12. Houseknecht K.L., Vanden Heuve J.P., Moya-Camarena S.Y., Portocarrero C.P., Peck L.W., Nickel K.P., Belury M.A. (1998): Dietary conjugated linoleic acid normalizes impaired glucose tolerance in the Zucker diabetic fatty FA/FA rat. Biochemical and Biophysical Research Communications, 244, 678-682. Go to original source... Go to PubMed...
    13. Koinarski V., Georgieva N.V., Gadjeva V., Petkov P. (2005): Antioxidant status of broiler chickens infected with Eimeria acervulina. Revue de Médecine Vétérinaire, 156, 498-502.
    14. Laurent F., Mancassola R., Lacroix S., Menezes R., Naciri M. (2001): Analysis of chicken mucosal immune response to Eimeria tenella and Eimeria maxima infection by quantitative reverse transcription-PCR. Infection and Immunity, 69, 2527-2534. Go to original source... Go to PubMed...
    15. Lillehoj H.S. (1989): Intestinal intraepithelial and splenic natural killer cell responses to eimerian infections in inbred chickens. Infection and Immunity, 57, 1879-1884. Go to original source... Go to PubMed...
    16. Lillehoj H.S. (1994): Analysis of Eimeria acervulina-induced changes in the intestinal T lymphocyte subpopulations in two chicken strains showing different levels of susceptibility to coccidiosis. Research in Veterinary Science, 56, 1-7. Go to original source... Go to PubMed...
    17. Lillehoj H.S., Choi K.D. (1998): Recombinant chicken interferon-gamma-mediated inhibition of Eimeria tenella development in vitro and reduction of oocyst production and body weight loss following Eimeria acervulina challenge infection. Avian Disease, 42, 307-314. Go to original source...
    18. Lillehoj H.S., Chung K.S. (1992): Postnatal development of T-lymphocyte subpopulations in the intestinal intraepithelium and lamina propria in chickens. Veterinary Immunology and Immunopathology, 31, 347-360. Go to original source... Go to PubMed...
    19. Lillehoj H.S., Min W., Dalloul R.A. (2004): Recent progress on the cytokine regulation of intestinal immune responses to Eimeria. Poultry Science, 83, 611-623. Go to original source... Go to PubMed...
    20. McCord J.M. (1983): The superoxide free radical: its biochemistry and pathophysiology. Surgery, 94, 412-414. Go to PubMed...
    21. Narciso-Gaytan C., Shin D., Sams A.R., Keeton J.T., Miller R.K., Smith S.B., Sanchez-Plata M.X. (2011): Lipid oxidation stability of omega-3- and conjugated linoleic acidenriched sous vide chicken meat. Poultry Science, 90, 473-480. Go to original source... Go to PubMed...
    22. NRC (1998): Nutrient Requirements of Swine. 10 th Ed. National Academies Press, Washington, USA.
    23. Palacios A., Piergiacomi V., Catala A. (2003): Antioxidant effect of conjugated linoleic acid and vitamin A during non enzymatic lipid peroxidation of rat liver microsomes and mitochondria. Molecular and Cellular Biochemistry, 250, 107-113. Go to original source... Go to PubMed...
    24. Sklan D., Melamed D., Friedman A. (1994): The effect of varying levels of dietary vitamin A on immune response in the chick. Poultry Science, 73, 843-847. Go to original source... Go to PubMed...
    25. Spitz D.R., Oberley L.W. (1989): An assay for superoxide dismutase activity in mammalian tissue homogenates. Analytical Biochemistry, 179, 8-18. Go to original source... Go to PubMed...
    26. Trout J.M., Lillehoj H.S. (1995): Eimeria acervulina infection: evidence for the involvement of CD8+ T lymphocytes in sporozoite transport and host protection. Poultry Science, 74, 1117-1125. Go to original source... Go to PubMed...
    27. van den Berg J.J.M., Cook N.E., Tribble D.L. (1995): Reinvestigation of the antioxidant properties of conjugated linoleic acid. Lipids, 30, 599-605. Go to original source... Go to PubMed...
    28. Wahle K.W.J., Heys S.D., Rotondo D. (2004): Conjugated linoleic acids: are they beneficial or detrimental to health? Progression in Lipid Research, 43, 553-587. Go to original source... Go to PubMed...
    29. Wills E.D. (1966): Mechanisms of lipid peroxide formation in animal tissues. Biochemistry Journal, 99, 667-676. Go to original source... Go to PubMed...
    30. Yamasaki M., Chujo H., Hirao A., Koyanagi N., Okamoto T., Tojo N., Oishi A., Iwata T., Yamauchi-Sato Y., Yamamoto T., Tsutsumi K., Tachibana H., Yamada K. (2003): Immunoglobulin and cytokine production from spleen lymphocytes is modulated in C57BL/6J mice by dietary cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid. Journal of Nutrition, 133, 784-788. Go to original source... Go to PubMed...
    31. Yu L. (2001): Free radical scavenging properties of conjugated linoleic acids. Journal of Agricultural and Food Chemistry, 49, 3452-3456. Go to original source... Go to PubMed...
    32. Yun C.H., Lillehoj H.S., Choi K.D. (2000): Eimeria tenella infection induces local gamma interferon production and intestinal lymphocyte subpopulation changes. Infection and Immunology, 68, 1282-1288. Go to original source... Go to PubMed...
    33. Zhang H.J., Guo Y.M., Tian Y.D., Yuan J.M. (2008): Dietary conjugated linoleic acid improves antioxidant capacity in broiler chicks. British Poultry Science, 49, 213-221. Go to original source... Go to PubMed...
    34. Zigterman G.J.W.J., van de Ven W., van Geffen C., Loeffen A.H.C., Panhuijzen J.H.M., Rijke E.O., Vermeulen A.N. (1993): Detection of mucosal immune responses in chickens after immunization or infection. Veterinary Immunology and Immunopathology, 36, 281-291. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content