Czech J. Anim. Sci., 2015, 60(7):302-310 | DOI: 10.17221/8276-CJAS

Optimum proportion of standardized ileal digestible sulfur amino acid to lysine to maximize the performance of 25-50 kg growing pigs fed reduced crude protein diets fortified with amino acidsOriginal Paper

G.J. Zhang1, P.A. Thacker2, J.K. Htoo3, S.Y. Qiao1
1 State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, P.R. China
2 Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Canada
3 Evonik Industries AG, Nutrition Research, Hanau-Wolfgang, Germany

The study was conducted to determine the standardized ileal digestible (SID) sulfur amino acid (SAA) to lysine (Lys) ratio required to maximize the performance of 25-50 kg pigs fed reduced crude protein (CP) diets fortified with crystalline amino acids. A total of 360 crossbred (Duroc × (Landrace × Large White)) pigs, weighing 25.6 ± 2.7 kg, were blocked by gender, litter, and initial body weight (BW) and allotted to 1 of 5 dietary treatments with 6 pens per treatment and 12 pigs per pen for a 35-day performance trial. The basal diet was based on corn, soybean meal, and wheat bran and was formulated to be deficient in SAA (50% proportion of SID SAA to Lys). Graded levels of dl-methionine were added to the basal diet at the expense of wheat bran in order to provide 55.6, 60.0, 65.6, or 70.0% proportion of SID SAA to Lys, respectively. A constant SID Lys level of 0.90% was set so that Lys was the second limiting amino acid (AA) in all diets. Average daily gain (ADG) and feed conversion ratio (FCR) improved (linear and quadratic, P < 0.05) with increasing dietary proportion of SID SAA to Lys. Increasing the dietary proportion of SID SAA to Lys decreased the serum urea nitrogen (SUN) level (quadratic, P < 0.05). A two-slope broken-line model estimated the optimum proportion of SID SAA to Lys to be 62.2, 61.5, and 62.3% for maximum ADG and minimum FCR and SUN, respectively, whereas a curvilinear-plateau model yielded an optimum proportion of SID SAA to Lys level of 63.8, 62.5, and 61.5% for maximum ADG and minimum FCR and SUN, respectively. Based on an average of these estimates, we conclude that the proportion of SID SAA to Lys required for 25-50 kg pigs fed low CP diets is 62.3%. This estimate is higher than the NRC (2012) recommendation of 56.1% for 25-50 kg pigs fed normal CP diets.

Keywords: amino acid ratio; requirement

Published: July 31, 2015  Show citation

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Zhang GJ, Thacker PA, Htoo JK, Qiao SY. Optimum proportion of standardized ileal digestible sulfur amino acid to lysine to maximize the performance of 25-50 kg growing pigs fed reduced crude protein diets fortified with amino acids. Czech J. Anim. Sci. 2015;60(7):302-310. doi: 10.17221/8276-CJAS.
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    References

    1. AOAC (2003): Official Methods of Analysis. 17 th Ed. Association of Official Analytical Chemists, Arlington, USA.
    2. Boisen S. (2003): Ideal dietary amino acid profiles for pigs. In: D'Mello J.P.F. (ed.): Amino Acids in Animal Nutrition. CABI Publishing, Edinburgh, UK, 157-186. Go to original source...
    3. Chen H.Y., Yi X.W., Zhang G.J., Lu N., Chu L.C., Thacker P.A., Qiao S.Y. (2011): Studies on reducing nitrogen excretion: 1. Net energy requirements of finishing pigs maximizing performance and carcass quality fed low crude protein diets supplemented with crystalline amino acids. Journal of Animal Science and Biotechnology, 2, 84-93.
    4. Chung T.K., Baker D.H. (1992): Ideal amino acid pattern for 10-kilogram pigs. Journal of Animal Science, 70, 3102-3111. Go to original source... Go to PubMed...
    5. Coma J., Carrion D., Zimmerman D.R. (1995): Use of plasma urea nitrogen as a rapid response criterion to determine the lysine requirement of pigs. Journal of Animal Science, 73, 472-476. Go to original source... Go to PubMed...
    6. D'Mello J.P.F. (2003): An outline of pathways in amino acid metabolism. In: D'Mello J.P.F. (ed.): Amino Acids in Animal Nutrition. CABI Publishing, Edinburgh, UK, 71-86. Go to original source...
    7. Gaines A.M., Yi G.F., Ratliff B.W., Srichana P., Alle G.L., Knight C.D., Perryman K.P. (2004): Estimation of the true ileal digestible sulfur amino acid : lysine ratio for growing pigs weighing 29-45 kilograms. Journal of Animal Science, 83 (Suppl. 1), 294.
    8. Gaines A.M., Yi G.F., Ratliff B.W., Srichana P., Kendall D.C., Allee G.L., Knight C.D., Perryman K.R. (2005): Estimation of the ideal ratio of true ileal digestible sulfur amino acids : lysine in 8- to 26-kg nursery pigs. Journal of Animal Science, 83, 2527-2534. Go to original source... Go to PubMed...
    9. Gillis A.M., Reijmers A., Pluske J.R., de Lange C.F.M. (2007): Influence of dietary methionine to methionine plus cysteine ratios on nitrogen retention in gilts fed purified diets between 40 and 80 kg live body weight. Canadian Journal of Animal Science, 87, 87-92. Go to original source...
    10. Jones B.N., Gilligan J.P. (1983): o-Phthalaldehyde precolumn derivatization and reversed phase high performance liquid chromatography of polypeptide hydrolysates and physiological fluids. Journal of Chromatography, 266, 471-482. Go to original source... Go to PubMed...
    11. Kerr B.J., Southern L.L., Bidner T.D., Friesen K.G., Easter R.A. (2003): Influence of dietary protein level, amino acid supplementation, and dietary energy levels on growingfinishing pig performance and carcass composition. Journal of Animal Science, 81, 3075-3087. Go to original source... Go to PubMed...
    12. Kim J.C., Mullan B.P., Frey B., Payne H.G., Pluske J.R. (2012): Whole body protein deposition and plasma amino acid profiles in growing and/or finishing pigs fed increasing levels of sulfur amino acids with and without Escherichia coli lipopolysaccharide challenge. Journal of Animal Science, 90, 362-365. Go to original source...
    13. Litvak N., Rakhshandeh A., Htoo J.K., de Lange C.F.M. (2013): Immune system stimulation increases the optimal dietary methionine to methionine plus cysteine ratio in growing pigs. Journal of Animal Science, 91, 4188-4196. Go to original source... Go to PubMed...
    14. Lu S.C. (2009): Regulation of glutathione synthesis. Molecular Aspects of Medicine, 30, 42-59. Go to original source... Go to PubMed...
    15. Malmezat T., Breuille D., Capitan P., Mirand P.P., Obled C. (2000): Glutathione turnover is increased during the acute phase of sepsis in rats. Journal of Nutrition, 130, 1239-1246. Go to original source... Go to PubMed...
    16. Noblet J., Fortune H., Shi X.S., Dubois S. (1994): Prediction of net energy value of feeds for growing pigs. Journal of Animal Science, 72, 344-354. Go to original source... Go to PubMed...
    17. NRC (2012): Nutrient Requirements of Swine. 11th Ed. The National Academies Press, Washington, USA.
    18. Peak S. (2005): TSAA requirement for nursery and growing pigs. In: Foxcroft G. (ed.): Advances in Pork Production. University of Alberta Press, Edmonton, Canada, 101-107.
    19. Pedersen C., Boisen S. (2001): Studies on the response time for plasma urea nitrogen as a rapid measure for dietary protein quality in pigs. Animal Science, 51, 209-216. Go to original source...
    20. Pomar C., Kyriazakis I., Emmans G.C., Knap P.W. (2003): Modeling stochasticity: dealing with populations rather than individual pigs. Journal of Animal Science, 81, E178-E186.
    21. Qiao S., Piao X., Feng Z., Ding Y., Yue L., Thacker P.A. (2008): The optimum methionine to methionine plus cystine ratio for growing pigs determined using plasma urea nitrogen and nitrogen balance. Asian-Australasian Journal of Animal Sciences, 21, 434-442. Go to original source...
    22. Rakhshandeh A., Htoo J.K., de Lange C.F.M. (2010): Immune system stimulation of growing pigs does not alter apparent ileal amino acid digestibility but reduces the ratio between whole body nitrogen and sulfur retention. Livestock Science, 134, 21-23. Go to original source...
    23. Reeds P.J., Fjeld C.R., Jahoor F. (1994): Do the differences between the amino acid compositions of acute-phase and muscle proteins have a bearing on nitrogen loss in traumatic states? Journal of Nutrition, 124, 906-910. Go to original source... Go to PubMed...
    24. Robbins K.R., Norton H.W., Baker D.H. (1979): Estimation of nutrient requirements from growth data. Journal of Nutrition, 109, 1710-1714. Go to original source... Go to PubMed...
    25. Robbins K.R., Saxton A.M., Southern L.L. (2006): Estimation of nutrient requirements using broken-line regression analysis. Journal of Animal Science, 84, E155-E165. Go to original source... Go to PubMed...
    26. Sauvant D., Perez J.M., Tran G. (eds) (2004): Tables of Composition and Nutritional Value of Feed Materials: Pigs, Poultry, Cattle, Sheep, Goats, Rabbits, Horses and Fish. INRA Editions, Paris, France. (in French) Go to original source...
    27. Sedgwick G.W., Fenton T.W., Thompson J.R. (1991): Effect of protein precipitating agents on the recovery of plasma free amino acids. Canadian Journal of Animal Science, 71, 953-957. Go to original source...
    28. Shoveller A.K., Brunton J.A., Pencharz P.B., Ball R.O. (2003): The methionine requirement is lower in neonatal piglets fed parentally than in those fed enterally. Journal of Nutrition, 133, 1390-1397. Go to original source... Go to PubMed...
    29. Tao Y., Ying C., Ni Z., Li J., Li L. (1982): Clinical Biochemical Analysis. Shanghai Scientific Technical Press, Shanghai, China.
    30. Wang T.C., Fuller. M.F. (1989): The optimum dietary amino acid pattern for growing pigs. British Journal of Nutrition, 62, 77-89. Go to original source... Go to PubMed...
    31. Wiltafsky M.K., Schmidtlein B., Roth F.X. (2009): Estimates of the optimum dietary ratio of standardized ileal digestible valine to lysine for eight to twenty-five kilograms of body weight pigs. Journal of Animal Science, 87, 2544-2553. Go to original source... Go to PubMed...
    32. Yi G.F., Gaines A.M., Ratliff B.W., Srichana P., Alle G.L., Knight C.D., Perryman K.P. (2005): Estimation of the true ileal digestible sulfur amino acid : lysine ratio for growing pigs weighing 28-49 kilograms. Journal of Animal Science, 83 (Suppl. 1), 213. Go to original source... Go to PubMed...
    33. Yi G.F., Gaines A.M., Ratliff B.W., Srichana P., Allee G.L., Perryman K.R., Knight C.D. (2006): Estimation of the true ileal digestible lysine and sulfur amino acid requirement and comparison of the bioefficacy of 2-hydroxy4-(methylthio) butanoic acid and dl-methionine in eleven- to twenty-six-kilogram nursery pigs. Journal of Animal Science, 84, 1709-1721. Go to original source... Go to PubMed...
    34. Zhang G.J., Song Q.L., Xie C.Y., Chu L.C., Thacker P.A., Htoo J.K., Qiao S.Y. (2012): Estimation of the ideal standardized ileal digestible tryptophan to lysine ratio for growing pigs fed low crude protein diets supplemented with crystalline amino acids. Livestock Science, 149, 260-266. Go to original source...
    35. Zhang G.J., Xie C.Y, Thacker P.A., Htoo J.K., Qiao S.Y. (2013): Estimation of the ideal ratio of standardized ileal digestible threonine to lysine for growing pigs (22-50 kg) fed low crude protein diets supplemented with crystalline amino acids. Animal Feed Science and Technology, 180, 83-91. Go to original source...

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