Czech J. Anim. Sci., 2015, 60(1):38-44 | DOI: 10.17221/7910-CJAS

Effects of dietary supplementation of chitosan on immune and antioxidative function in beef cattleOriginal Paper

T. Li*, R. Na*, P. Yu, B. Shi, S. Yan, Y. Zhao, Y. Xu
College of Animal Science, Inner Mongolia Agricultural University, Huhhot, P.R. China

The present experiment was conducted to study the effects of chitosan (CHI) on immune and antioxidative function in beef cattle. A total of 24 fattening Simmental cattle with similar body weight and age were divided randomly into three dietary groups, and the three diets contained 0, 500, and 1000 mg/kg CHI, respectively. The feeding trial lasted for 84 days. It was found that: (1) the addition of CHI in diets improved (P < 0.05) the levels of IgA and interleukin-1, and decreased (P < 0.1) the levels of soluble cluster of differentiation 4 receptor in serum at middle stage except that IgA remained unchanged in 1000 mg/kg CHI group. The levels of IgM and IgA tended to be increased (P < 0.1) by dietary CHI at later stage of the experiment; (2) the addition of 500 mg/kg CHI in diets increased (P < 0.1) total superoxide dismutase activity and decreased (P < 0.05) malondialdehyde content in serum at early and later stages, respectively. In conclusion, these results indicated that addition of 500 mg/kg CHI affected humoral and cellular immune responses, and improved the antioxidative function of beef cattle.

Keywords: dietary chitosan; Simmental cattle; humoral immunity; antioxidative enzyme; malondialdehyde

Published: January 31, 2015  Show citation

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Li T, Na R, Yu P, Shi B, Yan S, Zhao Y, Xu Y. Effects of dietary supplementation of chitosan on immune and antioxidative function in beef cattle. Czech J. Anim. Sci. 2015;60(1):38-44. doi: 10.17221/7910-CJAS.
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    References

    1. Afonso V., Champy R., Mitrovic D., Collin P., Lomri A. (2007): Reactive oxygen species and superoxide dismutases: role in joint diseases. Joint Bone Spine, 74, 324-329. Go to original source... Go to PubMed...
    2. Anandan R., Ganesan B., Obulesu T., Mathew S., Asha K.K., Lakshmanan P.T., Zynudheen A.A. (2013): Antiaging effect of dietary chitosan supplementation on glutathionedependent antioxidant system in young and aged rats. Cell Stress Chaperones, 18, 121-125. Go to original source... Go to PubMed...
    3. Cui J.J., Yuan J.F., Zhang Z.Q. (2010): Anti-oxidation activity of the crude polysaccharides isolated from Polygonum cillinerve (Nakai) Ohwi in immunosuppressed mice. Journal of Ethnopharmacology, 132, 512-517. Go to original source... Go to PubMed...
    4. David A.Z., Connie J.R., Kenneth W.H., Jeffrey S., John W.G. (2007): Chitosan solution enhances both humoral and cellmediated immune responses to subcutaneous vaccination. Vaccine, 11, 2085-2094. Go to original source... Go to PubMed...
    5. Esumi K., Takei N., Yoshimura T. (2003): Antioxidant-potentiality of gold-chitosan nanocomposites. Colloids and Surfaces B: Biointerfaces, 32, 117-123. Go to original source...
    6. Frey A., Di J.C., Zurakowski D. (1998): A statistically defined endpoint titer determination method for immunoassays. Journal of Immunological Methods, 221, 35-41. Go to original source... Go to PubMed...
    7. Guo Z.Y., Xing R.E., Liu S., Zhong Z.M., Li P.C. (2008): Synthesis and hydroxyl radicals scavenging activity of quaternized carboxymethyl chitosan. Carbohydrate Polymers, 73, 173-177. Go to original source...
    8. Johnson R.W. (1997): Inhibition of growth by pro-inflammatory cytokines: an integrated view. Journal of Animal Science, 75, 1244-1255. Go to original source... Go to PubMed...
    9. Kong X.F., Zhou X.L., Lian G.Q., Blachier F., Liu G., Tan B.E., Nyachoti C.M., Yin Y.L. (2014): Dietary supplementation with chitooligosaccharides alters gut microbiota and modifies intestinal luminal metabolites in weaned Huanjiang mini-piglets. Livestock Science, 160, 97-101. Go to original source...
    10. Li H.Y. (2009): Effects of chitosan on immune function in broilers and the underlying mechanisms. Ph.D. Thesis. Hohhot, Inner Mongolia Agricultural University. (in Chinese)
    11. Li H.Y., Yan S.M., Shi B.L. (2009): Effect of chitosan on nitric oxide content and inducible nitric oxide synthase activity in serum and expression of inducible nitric oxide synthase mRNA in small intestine of broiler chickens. Asian-Australasian Journal of Animal Sciences, 22, 1048-1053. Go to original source...
    12. Li J.L., Shi B.L., Yan S.M., Jin L., Guo Y.W., Xu Y.Q., Li T.Y., Guo X.Y. (2013): Effects of dietary supplementation of chitosan on humoral and cellular immune function in weaned piglets. Animal Feed Science and Technology, 186, 204-208. Go to original source...
    13. Lim B.O., Yamada K., Nonaka M., Kuramoto Y., Hung P., Sugano M. (1997): Dietary fibers modulate indices of intestinal immune function in rats. Journal of Nutrition, 127, 663-667. Go to original source... Go to PubMed...
    14. Liu M., Shi T., Liu J.Q. (2011): Effects of chitosan on antioxidative function and immune organ index in broilers. Journal of Chinese Feed, 15, 22-25. (in Chinese)
    15. Liu Q.H., Liang X.W., Wang G.L. (2007): Effects of dietary supplementation of chitosan on dry matter intake and blood parameters for dry dairy cows. Journal of Animal and Feed Sciences, 16, 430-435. Go to original source...
    16. McKee A.S., Munks M.W., Marrack P. (2007): How do adjuvants work? Important considerations for new generation adjuvants. Immunity, 27, 687-690. Go to original source... Go to PubMed...
    17. Metcalfe D.D. (1991): Food allergy. Current Opinion in Immunology, 3, 881-886. Go to original source... Go to PubMed...
    18. NRC (2000): Nutrient Requirements of Beef Cattle. 7th Ed. The National Academies Press, Washington, USA.
    19. Ouali A., Herrera-Mendez C.H., Coulis G., Becila S., Boudjellal A., Aubry L., Sentandreu M.A. (2006): Revisiting the conversion of muscle into meat and the underlying mechanisms. Meat Science, 74, 44-58. Go to original source... Go to PubMed...
    20. Ren H.J. (2008): Effects of chitosan on production performance and immune function in dairy cattle. Ph.D. Thesis. Hohhot, Inner Mongolia Agricultural University. (in Chinese)
    21. Renckens R., Roelofs J.J., Knapp S., de Vos A.F., Florquin S., van der Poll T. (2006): The acute-phase response and serum amyloid A inhibit the inflammatory response to Acinetobacter baumannii Pneumonia. Journal of Infectious Diseases, 193, 187-195. Go to original source... Go to PubMed...
    22. Schley P.D., Field C.J. (2002): The immune-enhancing effects of dietary fibres and prebiotics. British Journal of Nutrition, 87, S221-S230. Go to original source... Go to PubMed...
    23. Siwicki A.K., Anderson D.P., Rumsey G.L. (1994): Dietary intake of immunostimulants by rainbow trout affects nonspecific immunity and protection against furunculosis. Veterinary Immunology and Immunopathology, 41, 125-139. Go to original source... Go to PubMed...
    24. Song C.Y., Jiang J.Y., Han X.J., Shi J.S., Wei S.D. (2010): Effects of immunological stress on interleukin-1β, cortisol and growth hormone in different breed piglets. Reproduction and Physiology, 46, 19-22. (in Chinese)
    25. Sun T., Yao Q., Zhou D., Mao F. (2008): Antioxidant activity of N-carboxymethyl chitosan oligosaccharides. Bioorganic & Medicinal Chemistry Letters, 18, 5774-5776. Go to original source... Go to PubMed...
    26. Tokura S., Tamura H., Azuma I. (1999): Immunological aspects of chitin and chitin derivatives administered to animals. EXS, 87, 279-292. Go to original source... Go to PubMed...
    27. Tomida H., Fujii T., Furutani N., Michihara A., Yasufuku T., Akasaki K., Maruyama T., Otagiri M., Gebicki J.M., Anraku M. (2009): Antioxidant properties of some different molecular weight chitosans. Carbohydrate Research, 344, 1690-1696. Go to original source... Go to PubMed...
    28. Uehara S., Gothoh K., Handa H., Tomita H., Tomita Y. (2003): Immune function in patients with acute pancreatitis. Journal of Gastroenterology and Hepatology, 18, 363-370. Go to original source... Go to PubMed...
    29. Xiao D.F., Tang Z.R., Yin Y.L., Zhang B., Hu X.G., Feng Z.M., Wang J.Q. (2013): Effects of dietary administering chitosan on growth performance, jejunal morphology, jejunal mucosal sIgA, occludin, claudin-1 and TLR4 expression in weaned piglets challenged by enterotoxigenic Escherichia coli. International Immunopharmacology, 17, 670-676. Go to original source... Go to PubMed...
    30. Xu Y., Shi B., Yan S., Li J., Li T., Guo Y., Guo X. (2014): Effects of chitosan supplementation on the growth performance, nutrient digestibility, and digestive enzyme activity in weaned pigs. Czech Journal of Animal Science, 59, 156-163. Go to original source...
    31. Yin J., Wu M.M., Xiao H., Ren W.K., Duan J.L., Yang G., Li T.J., Yin Y.L. (2014): Development of an antioxidant system after early weaning in piglets. Journal of Animal Science, 92, 612-619. Go to original source... Go to PubMed...
    32. Yin Y.L., Tang Z.R., Sun Z.H., Liu Z.Q., Li T.J., Huang R.L., Ruan Z., Deng Z.Y., Gao B., Chen L.X., Wu G.Y., Kim S.W. (2008): Effect of galacto-mannan-oligosaccharides or chitosan supplementation on cytoimmunity and humoral immunity in early-weaned piglets. Asian-Australasian Journal of Animal Sciences, 21, 723-731. Go to original source...
    33. Yoon H.J., Moon M.E., Park H.S., Kim H.W., Im S.Y., Lee J.H., Kim Y.H. (2008): Effects of chitosan oligosaccharide (COS) on the glycerol-induced acute renal failure in vitro and in vivo. Food and Chemical Toxicology, 46, 710-716. Go to original source... Go to PubMed...
    34. Yu P., Zhao Y.G., Shi B.L., Yan S.M., Hong L., Xu Y.Q., Guo Y.W., Tian L.X. (2012): Effects of dietary supplementation of chitosan on growth performance and nutrient digestibility in beef cattle. Journal of Feed Industry, 33, 44-47. (in Chinese)
    35. Zhang J. (2012): Effects of dietary immunostimulants on growth, non-specific immune response and stress resistance of juvenile pacific white shrimp, Litopenaeus vannamei. Ph.D. Thesis. Guang Zhou, Sun Yat-sen University. (in Chinese)

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