Czech J. Anim. Sci., 2014, 59(11):519-528 | DOI: 10.17221/7733-CJAS

Mitochondrial DNA-based genetic evaluation of autochthonous cattle breeds in CroatiaOriginal Paper

A. Ivanković1, S. Paprika1, J. Ramljak1, P. Dovč2, M. Konjačić1
1 Department of Animal Production and Technology, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia
2 Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Domžale, Slovenia

Genetic diversity and phylogenetic relationship of three Croatian autochthonous cattle breeds was analyzed using a sequence of the mtDNA D-loop region. Among Busha, Istrian, and Slavonian Syrmian Podolian cattle 146 unrelated animals were tested. The sequencing of 780 base pairs of the mtDNA D-loop region revealed 39 polymorphic sites representing 28 different haplotypes. The highest numbers of haplotypes were observed in the Busha population and the lowest in the population of Slavonian Syrmian Podolian cattle, while the highest level of sequence diversity within a population was observed in the Istrian cattle. Our results indicate a high level of mtDNA diversity in the populations of Busha and Istrian cattle and a low level of preserved diversity in the population of Slavonian Syrmian Podolian cattle. The sequence analysis showed substantial subdivision between the breeds (FST = 0.1434), and a large fraction of variation within the breeds. Although the dominant haplotypes are classified as the T3 haplogroup, some of the haplotypes are classified as the rarer T2 and T5 haplogroups. Genetic information based on mtDNA typing has a great importance for the future conservation management and preservation of genetic diversity in autochthonous cattle breeds.

Keywords: mtDNA; genetic diversity; Bos taurus; phylogeny; conservation

Published: November 30, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ivanković A, Paprika S, Ramljak J, Dovč P, Konjačić M. Mitochondrial DNA-based genetic evaluation of autochthonous cattle breeds in Croatia. Czech J. Anim. Sci. 2014;59(11):519-528. doi: 10.17221/7733-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Achilli A., Bonfiglio S., Olivieri A., Malusa A., Pala M., Kashani B.H., Perego U.A., Ajmone-Marsan P., Liotta L., Semino O., Bandelt H.J., Ferretti L., Torroni A. (2009): The multifaceted origin of taurine cattle reflected by the mitochondrial genome. PLoS ONE, 4, e5753. Go to original source... Go to PubMed...
    2. Anderson S., de Bruijn M.H., Coulson A.R., Eperon I.C., Sanger F., Young I.G. (1982): Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome. Journal of Molecular Biology, 156, 683-717. Go to original source... Go to PubMed...
    3. Bonfiglio S., Achilli A., Olivieri A., Negrini R., Colli L., Liotta L., Ajmone-Marsan P., Torroni A., Ferretti L. (2010): The enigmatic origin of bovine mtDNA haplogroup R: Sporadic interbreeding or an independent event of Bos primigenius domestication in Italy? PLoS ONE, 5, e15760. Go to original source... Go to PubMed...
    4. FAO (2007): The State of the World's Animal Genetic Resources for Food and Agriculture - in Brief. Commission on Genetic Resources for Food and Agriculture, Food and Agriculture Organization of the United Nations, Rome, Italy.
    5. FAO (2009): Status and trends of animal genetic resources - 2008. In: Mtg. Intergovernmental Technical Working Group on Animal Genetic Resources for Food and Agriculture of the Commission on Genetic Resources for Food and Agriculture, 5th Session, Rome, Italy.
    6. Ivankovic A., Kelava N., Konjacic M., Caput P., Ramljak J. (2007): Exterior characteristics of Istrian cattle. Stočarstvo, 61, 161-173. (in Croatian)
    7. Kimura M. (1980): A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111-120. Go to original source... Go to PubMed...
    8. Maretto F., Ramljak J., Sbarra F., Penasa M., Mantovani R., Ivankovic A., Bittante G. (2012): Genetic relationships among Italian and Croatian Podolian cattle breeds assessed by microsatellite markers. Livestock Science, 150, 256-264. Go to original source...
    9. Medugorac I., Medugorac A., Russ I., Veit-Kensch C.E., Taberlet P., Luntz B., Mix H.M., Forster M. (2009): Genetic diversity of European cattle breeds highlights the conservation value of traditional unselected breeds with high effective population size. Molecular Ecology, 18, 394-410. Go to original source... Go to PubMed...
    10. Medugorac I., Veit-Kensch C.E., Ramljak J., Brka M., Markovic B., Stojanovic S., Bytyqi H., Kochoski L., Kume K., Grunenfelder H.P., Bennewitz J., Forster M. (2011): Conservation priorities of genetic diversity in domesticated metapopulations: a study in taurine cattle breeds. Ecology and Evolution, 1, 408-420. Go to original source... Go to PubMed...
    11. Ogrizek A. (1957): Contribution to the knowledge of Istrian cattle (Part 1). Acta Biologica I, 155-186. (in Croatian)
    12. Ramljak J., Ivankovic A., Veit-Kensch C.E., Forster M., Medugorac I. (2011): Analysis of genetic and cultural conservation value of three indigenous Croatian cattle breeds in a local and global context. Animal Breeding and Genetics, 128, 73-84. Go to original source... Go to PubMed...
    13. Saitou N., Nei M. (1987): The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406-425. Go to PubMed...
    14. Slatkin M. (1995): A measure of population subdivision based on microsatellite allele frequencies. Genetics, 134, 983-993. Go to original source... Go to PubMed...
    15. Smalcelj I., Rako A., Jelicic I. (1958): Development tendencies of the cattle production in Istria. Stočarstvo, 12, 1-15. (in Croatian)
    16. White P.S., Densmore L.D. (1992): Mitochondrial DNA Isolation: Molecular Genetic Analysis of Populations. A Practical Approach. Oxford University Press, Oxford, UK.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content