Czech J. Anim. Sci., 2014, 59(10):450-459 | DOI: 10.17221/7708-CJAS

Essential oils effect on rumen fermentation and biohydrogenation under in vitro conditionsOriginal Paper

M. Gunal1, A. Ishlak2, A.A. AbuGhazaleh2, W. Khattab3
1 Department of Animal Science, Süleyman Demirel University, Isparta, Turkey
2 Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, USA
3 Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Benha University Moshtohor Tukh, Qalubia, Egypt

The effects of adding essential oils (EO) at different levels (125, 250, 500 mg/l) on rumen fermentation and biohydrogenation were examined in a rumen batch culture study. Treatments were: control without EO (CON), control with anise oil (ANO), cedar wood oil (CWO), cinnamon oil (CNO), eucalyptus oil (EUO), and tea tree oil (TEO). Essential oils, each dissolved in 1 ml of ethanol, were added to the culture flask containing 40 ml of buffer solution, 2 ml of reduction solution, 10 ml of rumen fluid, 25 mg of soybean oil, and 0.5 g of the diet. After 24 h of incubation in a water batch at 39°C, three samples were collected from each flask and analyzed for ammonia-N, volatile fatty acids (VFA), and fatty acids (FA). Expect for CNO, the proportions of acetate, propionate, and acetate to propionate ratios were not affected (P > 0.05) by EO addition. Addition of CWO, CNO, and TEO reduced total VFA concentrations (P < 0.05) regardless of dose level. The ammonia-N concentration was greater in cultures incubated with EO regardless of dose level. Compared with the CON, the concentrations of C18:0 and trans C18:1 were reduced (P < 0.05) with EO addition regardless of dose level. Compared with the CON, the concentration of linoleic acid was greater (P < 0.05) when EO were added at 500 mg/l. EO tested in this study had no effects on VFA profile but significantly reduced the formation of biohydrogenation products (C18:0 and trans C18:1).

Keywords: batch culture; trans fatty acids; rumen

Published: October 31, 2014  Show citation

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Gunal M, Ishlak A, AbuGhazaleh AA, Khattab W. Essential oils effect on rumen fermentation and biohydrogenation under in vitro conditions. Czech J. Anim. Sci. 2014;59(10):450-459. doi: 10.17221/7708-CJAS.
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