Czech J. Anim. Sci., 2014, 59(8):374-380 | DOI: 10.17221/7588-CJAS
Susceptibility of Escherichia coli and Clostridium perfringens to sucrose monoesters of capric and lauric acidOriginal Paper
- 1 Institute of Animal Science, Prague-Uhříněves, Czech Republic
- 2 Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
- 3 Institute of Microbiology, The Academy of Sciences of the Czech Republic, Prague, Czech Republic
The sucrose monoesters of capric and lauric acid were tested for their antibacterial activity towards two foodborne enteropathogenic bacteria - Escherichia coli (CCM 3954 - serotype O6 and E22 - serotype O103) and Clostridium perfringens (CNCTC 5459 and CIP 105178). Antibacterial activity was evaluated by the plating technique. Sucrose monocaprate significantly decreased the number of viable cells of E. coli at all tested concentrations (0.1-5 mg/ml). The overnight incubation of C. perfringens with the sucrose ester of lauric acid at 0.1-5 mg/ml reduced the number of viable cells below the detection limit (2 log10 CFU/ml). Incubating E. coli CCM 3954 and C. perfringens CNCTC 5459 with monoesters (0.1 and 2 mg/ml) did not influence the K+ permeability of the cytoplasmic membrane in cells during a 2.5-minute treatment. A 30-minute incubation of E. coli CCM 3954 and C. perfringens CNCTC 5459 with esters (0.1 and 2 mg/ml) revealed damage to cytoplasmic structures, as observed by transmission electron microscopy.
Keywords: inhibition; ester; fatty acids; bacterium; antibacterial
Published: August 31, 2014 Show citation
ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Alakomi H.-L., Skytta E., Saarela M., Mattila-Sandholm T., Latva-Kala K., Helander I.M. (2000): Lactic acid permeabilizes Gram negative bacteria by disrupting the outer membrane. Applied and Environmental Microbiology, 66, 2001-2005.
Go to original source...
Go to PubMed...
- Boyaval P., Corre C., Dupuis C., Roussel E. (1995): Effects of free fatty acids on propionic acid bacteria. Lait, 75, 17-29.
Go to original source...
- Conley A.J., Kabara J.J. (1973): Antimicrobial action of esters of polyhydric alcohols. Antimicrobial Agents and Chemotherapy, 4, 501-506.
Go to original source...
Go to PubMed...
- Desbois A.P., Smith V.J. (2010): Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology, 85, 1629-1642.
Go to original source...
Go to PubMed...
- Ferrer M., Soliveri J., Plou F.J., Lopez-Cortes N., ReyesDuarte D., Christensen M., Copa-Patino J.L., Ballesteros A. (2005): Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida antarctica B, and their antimicrobial properties. Enzyme and Microbial Technology, 36, 391-398.
Go to original source...
- Fietcher A. (1992): Biosurfactants: moving towards industrial application. Trends in Biotechnology, 10, 208-217.
Go to original source...
Go to PubMed...
- Galbraith H., Miller T.B. (1973): Effect of long chain fatty acids on bacterial respiration and amino acid uptake. Journal of Applied Bacteriology, 36, 659-675.
Go to original source...
Go to PubMed...
- Godshall M.A. (2001): Future directions for the sugar industry. International Sugar Journal, 103, 378-384.
- Habulin M., Sabeder S., Knez Z. (2008): Enzymatic synthesis of sugar fatty acid esters in organic solvent and in supercritical carbon dioxide and their antimicrobial activity. The Journal of Supercritical Fluids, 45, 338-345.
Go to original source...
- Hathcox A.K., Beuchat L.R. (1996): Inhibitory effects of sucrose fatty acid esters, alone and in combination with ethylenediaminetetraacetic acid and other organic acids, on viability of Escherichia coli O157:H7. Food Microbiology, 13, 213-225.
Go to original source...
- Kabara J.J., Swieczkowski D.M., Conley A.J., Truant J.P. (1972): Fatty acids and derivatives as antimicrobial agents. Antimicrobial Agents and Chemotherapy, 2, 23-28.
Go to original source...
Go to PubMed...
- Karlova T., Polakova L., Smidrkal J., Filip V. (2010): Antimicrobial effects of fatty acid fructose esters. Czech Journal of Food Sciences, 28, 146-149.
Go to original source...
- Kato A., Arima K. (1971): Inhibitory effect of sucrose ester of lauric acid on the growth of Escherichia coli. Biochemical and Biophysical Research Communications, 42, 596-601.
Go to original source...
Go to PubMed...
- Katsu T., Tsuchiya T., Fujita Y. (1984): Dissipation of membrane potential of Escherichia coli cells induced by macromolecular polylysine. Biochemical and Biophysical Research Communications, 122, 401-406.
Go to original source...
Go to PubMed...
- Marounek M., Skrivanova E., Rada V. (2003): Susceptibility of Escherichia coli to C2-C18 fatty acids. Folia Microbiologica, 48, 731-735.
Go to original source...
Go to PubMed...
- Marounek M., Pavlata L., Misurova L., Volek Z., Dvorak R. (2012): Changes in the composition of goat colostrum and milk fatty acids during the first month of lactation. Czech Journal of Animal Science, 57, 28-33.
Go to original source...
- Molatova Z., Skrivanova E., Macias B., McEwan N.R., Brezina P., Marounek M. (2010): Susceptibility of Campylobacter jejuni to organic acids and monoacylglycerols. Folia Microbiologica, 55, 215-220.
Go to original source...
Go to PubMed...
- Monk J.D., Beuchat L.R., Hathcox A.K. (1996): Inhibitory effects of sucrose monolaurate, alone and in combination with organic acids, on Listeria monocytogenes and Staphylococcus aureus. Journal of Applied Microbiology, 81, 7-18.
Go to original source...
Go to PubMed...
- Nobmann P., Smith A., Dunne J., Henehan G., Bourke P. (2009): The antimicrobial efficacy and structure activity relationship of novel carbohydrate fatty acid derivatives against Listeria spp. and food spoilage microorganisms. International Journal of Food Microbiology, 128, 440-445.
Go to original source...
Go to PubMed...
- Nobmann P., Bourke P., Dunne J., Henehan G. (2010): In vitro antimicrobial activity and mechanism of action of novel carbohydrate fatty acid derivatives against Staphylococcus aureus and MRSA. Journal of Applied Microbiology, 108, 2152-2161.
Go to original source...
Go to PubMed...
- Ohmizo C., Yata M., Katsu T. (2004): Bacterial cytoplasmic membrane permeability assay using ion-selective electrodes. Journal of Microbiological Methods, 59, 173-179.
Go to original source...
Go to PubMed...
- Polakova L., Karlova T., Smidrkal J., Filip V. (2010): Enzymatic synthesis of fatty acid derivatives with antimicrobial activity and their use. Chemické listy, 104, 692-696.
- Polat T., Linhardt R.J. (2001): Syntheses and applications of sucrose-based esters. Journal of Surfactants and Detergents, 4, 415-421.
Go to original source...
- Ricke S.C. (2003): Perspectives on the use of organic acids and short chain fatty acids as antimicrobials. Poultry Science, 82, 632-639.
Go to original source...
Go to PubMed...
- Sheu C.W., Freese E. (1972): Effects of fatty acids on growth and envelope proteins of Bacillus subtilis. Journal of Bacteriology, 111, 516-524.
Go to original source...
Go to PubMed...
- Skrivanova E., Marounek M., Benda V., Brezina P. (2006): Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringens to organic acids and monolaurin. Veterinarni Medicina, 51, 81-88.
Go to original source...
- Smith A., Nobmann P., Henehan G., Bourke P., Dunne J. (2008): Synthesis and antimicrobial evaluation of carbohydrate and polyhydroxylated non-carbohydrate fatty acid ester and ether derivatives. Carbohydrate Research, 343, 2557-2566.
Go to original source...
Go to PubMed...
- Timbermont L., Lanckriet A., Dewulf J., Nollet N., Schwarzer K., Haesebrouck F., Ducatelle R., Van Immerseel F. (2010): Control of Clostridium perfringens-induced necrotic enteritis in broilers by target-released butyric acid, fatty acids and essential oils. Avian Pathology, 39, 117-121.
Go to original source...
Go to PubMed...
- Wojtczak L., Wieckowski M.R. (1999): The mechanisms of fatty acid-induced proton permeability on the inner mitochondrial membrane. Journal of Bioenergetics and Biomembranes, 31, 447-455.
Go to original source...
Go to PubMed...
- Yan Y., Bornscheuer U.T., Cao L., Schmid R.D. (1999): Lipase-catalysed solid-phase synthesis of sugar fatty acid esters. Enzyme and Microbial Technology, 25, 725-728.
Go to original source...
- Yang C.M., Luedecke L.O., Swanson B.G., Davidson P.M. (2003): Inhibition of microorganisms in salad dressing by sucrose and methylglucose fatty acid monoesters. Journal of Food Processing and Preservation, 27, 285-298.
Go to original source...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.