Czech J. Anim. Sci., 2014, 59(7):297-301 | DOI: 10.17221/7526-CJAS

Identification of SNPs in ME1 gene and association analysis with meat quality traits in Chinese Red cattleOriginal Paper

G.L. Zhou1, Y. Cao2, H.G. Jin2
1 College of Life Science, Liaocheng University, Liaocheng, P.R. China
2 Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Gongzhuling, P.R. China

The objective of this study was to identify single nucleotide polymorphisms (SNPs) in coding regions of bovine ME1 gene and to evaluate if the polymorphisms are associated with meat quality traits in Chinese Red cattle. Four SNPs were identified: NW_001495544:g.1721768G>A in exon 2, g.1653796T>A in exon 4, g.1649532G>A in exon 5, and g.1546272T>C in exon 12 and they were genotyped by applying the PCR-RFLP method. Statistical analysis showed that two SNPs, g.1649532G>A and g.1546272T>C, were significantly associated with cooking loss and pH24h (P < 0.05). But no statistically significant differences were observed in the g.1721768G>A and g.1653796A>T SNPs for meat quality traits tested in Chinese Red cattle. This suggests that ME1 gene is a candidate that may have effects on meat quality traits in cattle.

Keywords: candidate gene; polymorphism; beef; Chinese Red cattle

Published: July 31, 2014  Show citation

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Zhou GL, Cao Y, Jin HG. Identification of SNPs in ME1 gene and association analysis with meat quality traits in Chinese Red cattle. Czech J. Anim. Sci. 2014;59(7):297-301. doi: 10.17221/7526-CJAS.
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    References

    1. Allais S., Leveziel H., Payet-Duprat N., Hocquette J.F., Lepetit J., Rousset S., Denoyelle C., Bernard-Capel C., Journaux L., Bonnot A., Renand G. (2010): The two mutations, Q204X and nt821, of the myostatin gene affect carcass and meat quality in young heterozygous bulls of French beef breeds. Journal of Animal Science, 88, 446-454. Go to original source... Go to PubMed...
    2. Allais S., Journaux L., Leveziel H., Payet-Duprat N., Raynaud P., Hocquette J.F., Lepetit J., Rousset S., Denoyelle C., Bernard-Capel C., Renand G. (2011): Effects of polymorphisms in the calpastatin and µ-calpain genes on meat tenderness in 3 French beef breeds. Journal of Animal Science, 89, 1-11. Go to original source... Go to PubMed...
    3. Capon F., Allen M.H., Ameen M., Burden A.D., Tillman D., Barker J.N., Trembath R.C. (2004): A synonymous SNP of the corneodesmosin gene leads to increased mRNA stability and demonstrates association with psoriasis across diverse ethnic groups. Human Molecular Genetics, 13, 2361-2368. Go to original source... Go to PubMed...
    4. Casas E., Shackelford S.D., Keele J.W., Koohmaraie M., Smith T.P.L., Stone R.T. (2003): Detection of quantitative trait loci for growth and carcass composition in cattle．Journal of Animal Science, 81, 2973-2983. Go to original source... Go to PubMed...
    5. Chang G.G., Tong L. (2003): Structure and function of malic enzymes, a new class of oxidative decarboxylases. Biochemistry, 42, 12721-12733. Go to original source... Go to PubMed...
    6. Gill J.L., Bishop S.C., McCorquodale C., Williams J.L., Wiener P. (2012): Identification of polymorphisms in the malic enzyme 1, NADP(+)-dependent, cytosolic and nuclear receptor subfamily 0, group B, member 2 genes and their associations with meat and carcass quality traits in commercial Angus cattle. Animal Genetics, 43, 88-92. Go to original source... Go to PubMed...
    7. Guay C., Madiraju S.R., Aumais A., Joly E., Prentki M. (2007): A role for ATP-citrate lyase, malic enzyme, and pyruvate/citrate cycling in glucose-induced insulin secretion. Journal of Biological Chemistry, 282, 35657-35665. Go to original source... Go to PubMed...
    8. Kimchi-Sarfaty C., Oh J.M., Kim I.W., Sauna Z.E., Calcagno A.M., Ambudkar S.V., Gottesman M.M. (2007): A "silent"polymorphism in the MDR1 gene changes substrate specificity. Science, 315, 525-528. Go to original source... Go to PubMed...
    9. Li C.J., Wang H.J., Li C.J., Zhou X. (2010): Variations in reproductive hormones during estrus cycle in local beef cattle from Jilin of China. Animal Husbandry and Feed Science, 2, 14-17.
    10. Monin G., Sellier P. (1985): Pork of low technological quality with a normal rate of muscle pH fall in the immediate post-mortem period: the case of the Hampshire breed. Meat Science, 13, 49-63. Go to original source... Go to PubMed...
    11. Mourot J., Kouba M. (1999): Development of intra- and intermuscular adipose tissue in growing Large White and Meishan pigs. Reproduction Nutrition Development, 39, 125-132. Go to original source... Go to PubMed...
    12. Nackley A.G., Shabalina S.A., Tchivileva I.E., Satterfield K., Korchynskyi O., Makarov S.S., Maixner W., Diatchenko L. (2006): Human catechol-O-methyltransferase haplotypes modulate protein expression by altering mRNA secondary structure. Science, 314, 1930-1933. Go to original source... Go to PubMed...
    13. Pannier L., Sweeney T., Hamill R.M., Ipek F., Stapleton P.C., Mullen A.M. (2009): Lack of an association between single nucleotide polymorphisms in the bovine leptin gene and intramuscular fat in Bos taurus cattle. Meat Science, 81, 731-737. Go to original source... Go to PubMed...
    14. Pearson A.M., Young R.B. (1989): Muscle and Meat Biochemistry. Academic Press Inc., San Diego, USA.
    15. Rhodri S., Deane C.M. (2010): Synonymous codon usage influences the local protein structure observed. Nucleic Acids Research, 38, 6719-6728. Go to original source... Go to PubMed...
    16. Vidal O., Varona L., Oliver M.A., Noguera J.L., Sanchez A., Amills M. (2006): Malic enzyme 1 genotype is associated with backfat thickness and meat quality traits in pigs. Animal Genetics, 37, 28-32. Go to original source... Go to PubMed...
    17. Zhou G.L., Zhang G.L., Cao Y., Jin H.G. (2011): Cloning and polymorphisms of the 3' untranslated region of malic enzyme gene in Chinese Red cattle. Meat Science, 89, 72-75. Go to original source... Go to PubMed...

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