Czech J. Anim. Sci., 2012, 57(9):410-419 | DOI: 10.17221/6315-CJAS

Effects of a rumen-protected form of methionine and a methionine analogue on the lactation performance of dairy cows

J. Čermáková, V. Kudrna, J. Illek, K. Blažková, J. Haman
Institute of Animal Science, Prague-Uhříněves, Czech Republic

The objective the present study was to determine the influence of a supplemental methionine analogue, the isopropyl ester of 2-hydroxy-4-(methylthio)-butanoic acid, commercially available as MetaSmartTM, on lactation performance, particularly milk protein production. The effects of this preparation were compared with those of a rumen-protected form of methionine, marketed as SmartamineTM M. Experiments were conducted according to a 3 × 3 Latin square design and included 30 high-yielding dairy cows (22 Holstein and 8 Czech Fleckvieh) randomly allocated to three balanced groups. Cows were fed a basal diet based on maize silage, lucerne silage, lucerne hay, fresh brewer's grains, and a concentrate mixture in the form of a total mixed ration ad libitum. The diet M was supplemented with MetaSmartTM (42.5 g/day) and diet S was supplemented with SmartamineTM M (19 g/day), while control diet C contained solvent-extracted soybean meal, which was added to achieve required levels of dietary protein. Each period lasted four weeks in total, including three preliminary weeks and one experimental week during which samples of milk and tail vein blood were taken. Supplementation of MetaSmartTM decreased dry matter intake of cows (18.96 kg) in contrast to the diet containing SmartamineTM M, for which dry matter intake was the highest (20.48 kg; P < 0.001). Despite decreased dry matter intake, the highest average milk yields were recorded for cows supplemented with MetaSmartTM (31.34 kg), which produced by approximately 1.14 kg (P < 0.001) and 0.78 kg (P < 0.01) more milk than cows fed diets C and S, respectively. As expressed by greater ratios milk/DMI, FCM/DMI, and ECM/DMI, the feed efficiency was improved in cows supplemented with MetaSmartTM.Both MetaSmartTM and SmartamineTM M dietary supplementation increased milk yield, milk protein concentrations, and yields and increased the prevalence of β-casein fraction in milk protein.

Keywords: nutrition; amino acids; milk production; milk protein; casein

Published: September 30, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Čermáková J, Kudrna V, Illek J, Blažková K, Haman J. Effects of a rumen-protected form of methionine and a methionine analogue on the lactation performance of dairy cows. Czech J. Anim. Sci. 2012;57(9):410-419. doi: 10.17221/6315-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. AOAC (1995): Official Methods of Analyses. 16th Ed. Association of Official Analytical Chemists, Arlington, USA.
    2. AOAC (2000): Official Methods of Analyses. 17th Ed. Association of Official Analytical Chemists, Washington, USA.
    3. AOAC (2005): Official Methods of Analyses. 18th Ed. Association of Official Analytical Chemists, Gaithersburg, USA.
    4. Baudet H.M. (1995): Nouvelles tables INRA. Alimentation, 228, 119-121.
    5. Broderick G.A., Muck R.E. (2009): Effect of alfalfa silage storage structure and rumen-protected methionine on production in lactating dairy cows. Journal of Dairy Science, 92, 1281-1289. Go to original source... Go to PubMed...
    6. Broderick G.A., Stevenson M.J., Patton R.A., Lobos N.E., Olmos Colmenero J.J. (2008): Effect of supplementing rumen-protected methionine on production and nitrogen excretion in lactating dairy cows. Journal of Dairy Science, 91, 1092-1102. Go to original source... Go to PubMed...
    7. Chen Z.H., Broderick G.A., Luchini N.D., Sloan B.K., Devillard E. (2011): Effect of feeding different sources of rumen-protected methionine on milk production and N-utilization in lactating dairy cows. Journal of Dairy Science, 94, 1978-1988. Go to original source... Go to PubMed...
    8. Dinn N.E., Shelford J.A., Fisher L.J. (1998): Use of the Cornell Net Carbohydrate and Protein System and rumen-protected lysine and methionine to reduce nitrogen excretion from lactating dairy cows. Journal of Dairy Science, 81, 229-237. Go to original source... Go to PubMed...
    9. Graulet B., Richard C., Robert J.C. (2004): The isopropyl ester of methionine hydroxy-analogue is absorbed through the rumen wall in the cow. Journal of Animal and Feed Sciences, 13 (Suppl.1), 269-272. Go to original source...
    10. Graulet B., Richard C., Robert J.C. (2005): Methionine availability in plasma of dairy cows supplemented with methionine hydroxy analog isopropyl ester. Journal of Dairy Science, 88, 3640-3649. Go to original source... Go to PubMed...
    11. Huber J.T., Emery R.S., Bergen W.G., Liesman J.S., Kung Jr. L., King K.J., Gardner R.W., Checketts M. (1984): Influences of methionine hydroxy analog on milk and milk fat production, blood serum lipids, and plasma amino acids. Journal of Dairy Science, 67, 2525-2531. Go to original source... Go to PubMed...
    12. INRA (2007): Alimentation des bovins, ovins et caprins: Besoins des animaux, valeurs des aliments: Tables INRA 2007. Quae Editions, France, 307 p.
    13. Johnson H.E., Whitehouse N.L., Garthwaite B.D., Piepenbrink M.S., Schwab C.G. (1999): Supplementation of liquid methionine analog (HMB) to barley and corn based diets for late gestation and early lactation multiparous Holstein cows. Journal of Dairy Science, 82 (Suppl. 1), 65.
    14. Kudrna V., Illek J., Marounek M., Nguyen Ngoc A. (2009): Feeding ruminally protected methionine to pre- and postpartum dairy cows: effect on milk performance, milk composition and blood parameters. Czech Journal of Animal Science, 54, 395-402. Go to original source...
    15. Lundquist R.G., Otterby D.E., Linn G.G. (1985): Influence of three concentrations of dl-methionine or methionine hydroxyl analogue on milk yield and composition. Journal of Dairy Science, 68, 3350-3354. Go to original source... Go to PubMed...
    16. Mertens D.R. (2002): Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. Journal of AOAC International, 85, 1217-1240. Go to PubMed...
    17. Noftsger S., St-Pierre N.R. (2003): Supplementation of methionine and selection of highly digestible rumen undegradable protein to improve nitrogen efficiency for milk production. Journal of Dairy Science, 86, 958-969. Go to original source... Go to PubMed...
    18. Noftsger S., St-Pierre N.R., Sylvester J.T. (2005): Determination of rumen degradability and ruminal effects of three sources of methionine in lactating cows. Journal of Dairy Science, 88, 223-237. Go to original source... Go to PubMed...
    19. NRC (2001): Nutrient requirements of dairy cattle. 7 th Ed. National Academy Press, Washington, USA.
    20. Ordway R.S., Boucher S.E., Whitehouse N.L., Schwab G.C., Sloan B.K. (2009): Effects of providing two forms of supplemental methionine to periparturient Holstein dairy cows on feed intake and lactational performance. Journal of Dairy Science, 92, 5154-5166. Go to original source... Go to PubMed...
    21. Patton R.A. (2010): Effect of rumen-protected methionine on feed intake, milk production, true milk protein concentration, and true milk protein yield, and the factors that influence these effects: a meta-analysis. Journal of Dairy Science, 93, 2105-2118. Go to original source... Go to PubMed...
    22. Phipps R.H., Reynolds C.K., Givens D.I., Jones A.K., Geraert P.A., Devillard E., Bennett R. (2008): Short communication: effects of 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester on milk production and composition of lactating Holstein dairy cows. Journal of Dairy Science, 91, 4002-4005. Go to original source... Go to PubMed...
    23. Piepenbrink M.S, Schingoethe D.J., Brouk M.J., Stegeman G.A. (1998): Systems to evaluate the protein quality of diets fed to lactating cows. Journal of Dairy Science, 81, 1046-1061. Go to original source... Go to PubMed...
    24. Pisulewski P.M., Kowalski Z.M. (1999): The effect of protected methionine on milk yield and its composition in lactating dairy cows fed grass silage-based diets. Journal of Animal and Feed Sciences, 8, 355-366. Go to original source...
    25. Robert J.C., Bennett R., Gros G. (2000): A method for supplying bioavailable methionine to a cow. World Intellectual Property Organization, International Publication No. WO 00/28835.
    26. Schwab C.G., Ordway R.S., Whitehouse N.L., Mesbah M.M.A. (1992a): Amino acid limitation and flow to the duodenum at four stages of lactation. 1. Sequence of lysine and methionine limitation. Journal of Dairy Science, 75, 3486-3502. Go to original source... Go to PubMed...
    27. Schwab C.G., Bozak C.K., Whitehouse N.L., Olson V.M. (1992b): Amino acid limitation and flow to the duodenum at four stages of lactation. 2. Extent of lysine and methionine limitation. Journal of Dairy Science, 75, 3503-3518. Go to original source... Go to PubMed...
    28. Schwab C.G., Bozak C.K., Whitehouse N.L. (2003): Amino acid balancing in the context of MP and RUP requirements. In: Proc. Four-State Applied Nutrition and Management Conference. Lacrosse, USA, 25-34..
    29. Socha M.T., Putnam D.E., Garthwaite B.D., Whitehouse N.L., Kierstead N.A., Schwab C.G., Ducharme G.A., Robert J.C. (2005): Improving intestinal amino acid supply of pre- and postpartum dairy cows with rumenprotected methionine and lysine. Journal of Dairy Science, 88, 1113-1126. Go to original source... Go to PubMed...
    30. Stokes M.R., Clark J.H., Steinmetz L.M. (1981): Performance of lactating dairy cows fed methionine or methionine analogue at two concentrations of dietary crude protein. Journal of Dairy Science, 64, 1686-1694. Go to original source...
    31. St-Pierre N.R., Sylvester J.T. (2005): Effects of 2-hydroxy4-(methylthio) butanoic acid (HMB) and its isopropyl ester on milk production and composition by Holstein cows. Journal of Dairy Science, 88, 2487-2497. Go to original source... Go to PubMed...
    32. Xu S., Harrison J.H., Chalupa W., Sniffen C., Julien W., Sato H., Fujieda T., Watanabe K., Ueda T., Suzuki H. (1998): The effect of ruminal bypass lysine and methionine on milk yield and composition of lactating cows. Journal of Dairy Science, 81, 1062-1077. Go to original source... Go to PubMed...
    33. Yang W.R., Sun H., Wang Q.Y., Liu F.X., Yang Z.B. (2010): Effects of rumen-protected methionine on dairy performance and amino acid metabolism in lactating cows. American Journal of Animal and Veterinary Sciences, 5, 1-7. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content