Czech J. Anim. Sci., 2012, 57(2):45-53 | DOI: 10.17221/5131-CJAS

The association of five polymorphisms with milk production traits in Czech Fleckvieh cattle

J. Boleckova, J. Matejickova, M. Stipkova, J. Kyselova, L. Barton
Institute of Animal Science, Prague-Uhříněves, Czech Republic

The aim of this study was to estimate allelic and genotypic frequencies of five DNA markers that are positional and functional candidates for milk production traits in Czech Fleckvieh cattle. In addition, we evaluated the association of these markers with milk production traits and breeding values for milk production traits and also estimated linkage disequilibrium (LD) between two markers within the prolactin (PRL) gene. As part of this study, 505 Czech Fleckvieh cows were genotyped. The markers in proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), secreted phosphoprotein (SPP1), cytochrome P450 family 11 subfamily B hydroxylase (CYP11B1), and the two polymorphisms in the prolactin gene (PRL) showed evidence of segregation in our study. The PPARGC1A polymorphism was associated with milk yield, milk fat and protein traits. The polymorphism in SPP1 was significantly associated with milk protein percentage. The CYP11B1 polymorphism showed positive associations with milk composition traits and breeding values for milk yield, milk fat, and protein traits. Both polymorphisms within the PRL gene were associated with milk yield, milk fat and milk protein yield (individually and grouped). Linkage disequilibrium between the two polymorphisms in PRL was not observed. In conclusion, all markers examined in this study are important markers for milk production traits in Czech Fleckvieh cattle, and both markers within the PRL gene should be evaluated in future research.

Keywords: dairy cattle; PPARGC1A; CYP11B1; PRL; SPP1; milk yield; milk composition

Published: February 29, 2012  Show citation

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Boleckova J, Matejickova J, Stipkova M, Kyselova J, Barton L. The association of five polymorphisms with milk production traits in Czech Fleckvieh cattle. Czech J. Anim. Sci. 2012;57(2):45-53. doi: 10.17221/5131-CJAS.
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    References

    1. Alipanah M., Kalashnikova L.A., Rodionov G.V. (2007): Polymorphism prolactin loci in Russian cattle. Journal of Animal and Veterinary Advances, 6, 813-815. Go to original source...
    2. Benjamini Y., Hochber Y. (1995): Controlling the false discovery rate: A practical and powerful approach to multiple testing. Journals of the Royal Statistical Society, Series B, 57, 289-300. Go to original source...
    3. Benton C.R., Wright D.C., Bonen A. (2008): PGC-1 alpha-mediated regulation of gene expression and metabolism: implications for nutrition and exercise prescriptions. Applied Physiology, Nutrition, and Metabolism, 33, 843-862. Go to original source... Go to PubMed...
    4. Bionaz M., Loor J.J. (2008): Gene networks driving bovine milk fat synthesis during the lactation cycle. [online] BMC Genomics, 9, 366. doi: 10.1186/1471-2164-9-366. Go to original source... Go to PubMed...
    5. Bole-Feysot C., Goffin V., Edery M., Binart N., Kelly P.A. (1998): Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocrine Reviews, 19, 225-268. Go to original source... Go to PubMed...
    6. Brettes J.P., Mathelin C. (2008): Effet dual des androgènes sur la glande mammaire. Bulletin du Cancer (Paris), 95, 495-502. Go to PubMed...
    7. Brym P., Kaminski S., Wojcik E. (2005): Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits. Journal of Applied Genetics, 46, 179-185. Go to PubMed...
    8. Brym P., Malewski T., Starzynski R., Flisikowski K., Wojcik E., Rusc A., Zwierzchowski L., Kaminski S. (2007): Effect of new SNP within bovine prolactin gene enhancer region on expression in the pituitary gland. Biochemical Genetics, 45, 743-754. Go to original source... Go to PubMed...
    9. Chrenek P., Vašíček D., Bauerová M., Bulla J. (1998): Simultaneous analysis of bovine growth hormone and prolactin alleles by multiplex PCR and RFLP. Czech Journal of Animal Science, 43, 53-55.
    10. Chrenek P., Huba J., Vašíček D., Peskovicová D., Bulla J. (2003): The relation between genetic polymorphism markers and milk yield in brown Swiss cattle imported to Slovakia. Asian-Australasian Journal of Animal Sciences, 16, 1397-1401. Go to original source...
    11. Čítek J., Řehout V., Neubauerová V. (2001): Allele frequency at PRL (prolactin) and LGB (lactoglobulin beta) genes in Red cattle breeds from Central Europe and in other breeds. Czech Journal of Animal Science, 46, 433-438.
    12. Dybus A., Grzesiak W., Kamieniecki H., Szatkowska I., Sobek Z., Blaszczyk P., Czerniawska-Piatkowska E., Zych S., Muszynska M. (2005): Association of genetic variants of bovine prolactin with milk production traits of Black-and-White and Jersey cattle. Archiv für Tierzucht, 48, 149-156. Go to original source...
    13. Huang Q. (2003): EMLD [Program] [online]. Available from https://cge.mdanderson.org/_qhuang/Software/pub.htm (accessed May 12, 2011).
    14. Kaupe B., Kollers S., Fries R., Erhardt G. (2004): Mapping of CYP11B and a putative CYHR1 paralogous gene to bovine chromosome 14 by FISH. Animal Genetics, 35, 478-479. Go to original source... Go to PubMed...
    15. Kaupe B., Brandt H., Prinzenberg E.-M., Erhardt G. (2007): Joint analysis of the influence of CYP11B1 and DGAT1 genetic variation on milk production, somatic cell score, conformation, reproduction, and productive lifespan in German Holstein cattle. Journal of Animal Science, 85, 11-21. Go to original source... Go to PubMed...
    16. Khatami S.R., Lazebny O.E., Maksimenko V.F., Sulimova G.E. (2005): Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black-and-White cattle. Russian Journal of Genetics, 41, 167-173. (in Russian) Go to original source...
    17. Khatib H., Zaitoun I., Wiebelhaus-Finger J., Chang Y.M., Rosa G.J. (2007): The association of bovine PPARGC1A and OPN genes with milk composition in two independent Holstein cattle populations. Journal of Dairy Science, 90, 2966-2970. Go to original source... Go to PubMed...
    18. Komisarek J., Dorynek Z. (2009): Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls. Journal of Applied Genetics, 50, 125-132. Go to original source... Go to PubMed...
    19. Kowalewska-Luczak I., Kulig H., Kmiec M. (2010): Associations between the bovine PPARGC1A gene and milk production traits. Czech Journal of Animal Science, 55, 195-199. Go to original source...
    20. Leonard S., Khatib H., Schutzkus V., Chang Y.M., Maltecca C. (2005): Effects of the osteopontin gene variants on milk production traits in dairy cattle. Journal of Dairy Science, 88, 4083-4086. Go to original source... Go to PubMed...
    21. Lü A., Hu X., Chen H., Jiang J., Zhang C., Xu H., Gao X. (2010): Single nucleotide polymorphisms in bovine PRL gene and their associations with milk production traits in Chinese Holsteins. Molecular Biology Reports, 37, 547-551. Go to original source... Go to PubMed...
    22. Mehmannavaz Y., Amirinia C., Bonyadi M., Torshizi R.V. (2009): Effects of bovine prolactin gene polymorphism within exon 4 on milk related traits and genetic trends in Iranian Holstein bulls. African Journal of Biotechnology, 8, 4797-4801.
    23. Mitra A., Schlee P., Balakrishnan C.R., Pirchner F. (1995): Polymorphisms at growth-hormone and prolactin loci in Indian cattle and buffalo. Journal of Animal Breeding and Genetics, 112, 71-74. Go to original source...
    24. Nemir M., Bhattacharyya D., Li X., Singh K., Mukherjee A.B., Mukherjee B.B. (2000): Targeted inhibition of osteopontin expression in the mammary gland causes abnormal morphogenesis and lactation deficiency. The Journal of Biological Chemistry, 275, 969-976. Go to original source... Go to PubMed...
    25. Oztabak K., Un C., Tesfaye D., Akis I., Mengi A. (2008): Genetic polymorphisms of osteopontin (OPN), prolactin (PRL) and pituitary-specific transcript factor-1 (PIT-1) in South Anatolian and East Anatolian Red cattle. Acta Agriculturae Scandinavica, Section A - Animal Science, 58, 109-112. Go to original source...
    26. R Development Core Team (2010): R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available from http://www.R-project.org/
    27. Sasavage N.L., Nilson J.H., Horowitz S., Rottman F.M. (1982): Nucleotide sequence of bovine prolactin messenger RNA. Evidence for sequence polymorphism. The Journal of Biological Chemistry, 257, 678-681. Go to original source... Go to PubMed...
    28. Schennink A., Bovenhuis H., Léon-Kloosterziel K.M., van Arendonk J.A., Visker M.H. (2009): Effect of polymorphisms in the FASN, OLR1, PPARGC1A, PRL and STAT5A genes on bovine milk-fat composition. Animal Genetics, 40, 909-916. Go to original source... Go to PubMed...
    29. Sheehy P.A., Riley L.G., Raadsma H.W., Williamson P., Wynn P.C. (2009): A functional genomics approach to evaluate candidate genes located in a QTL interval for milk production traits on BTA6. Animal Genetics, 40, 492-498. Go to original source... Go to PubMed...
    30. Sørensen E.S., Petersen T.E. (1993): Purification and characterization of three proteins isolated from the proteose peptone fraction of bovine milk. Journal of Dairy Science, 60, 189-197. Go to original source... Go to PubMed...
    31. Storey J.D., Tibshirani R. (2003): Statistical significance for genomewide studies. Proceedings of the National Academy of Sciences USA, 100, 9440-9445. Go to original source... Go to PubMed...
    32. Weikard R., Kühn C., Goldammer T., Freyer G., Schwerin M. (2005): The bovine PPARGC1A gene: molecular characterization and association of an SNP with variation of milk fat synthesis. Physiological Genomics, 21, 1-13. Go to original source... Go to PubMed...
    33. White S.N., Casas E., Allan M.F., Keele J.W., Snelling W.M., Wheeler T.L., Shackelford S.D., Koohmaraie M., Smith T.P.L. (2007): Evaluation in beef cattle of six deoxyribonucleic acid markers developed for dairy traits reveals an osteopontin polymorphism associated with postweaning growth. Journal of Animal Science, 85, 1-10. Go to original source... Go to PubMed...
    34. Wibowo T.A., Gaskins C.T., Newberry R.C., Thorgaard G.H., Michal J.J., Jiang Z. (2008): Genome assembly anchored QTL map of bovine chromosome 14. International Journal of Biological Sciences, 4, 406-414. Go to original source... Go to PubMed...
    35. Wu Z., Puigserver P., Andersson U., Zhang C., Adelmant G., Mootha V., Troy A., Cinti S., Lowell B., Scarpulla R.C., Spiegelman B.M. (1999): Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1. Cell, 98, 115-124. Go to original source... Go to PubMed...

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