Czech J. Anim. Sci., 2011, 56(3):99-106 | DOI: 10.17221/56/2010-CJAS

Effects of a probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats

L. Strojný1, A. Bomba1, E. Hijová1, A. Chmelárová1, G. Mojžišová1, I. Bertková1, J. Koprovičová1, M. Pomfy2, V. Strompfová3, M. Molokáčová4
1 Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
2 Institute of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
3 Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic
4 Department of Microbiology, Teaching Hospital, Košice, Slovak Republic

Effects of the probiotic (PRO) Lactobacillus plantarum and of the combination of PRO and the prebiotic (PRE) inulin enriched with oligofructose (2%), and PRO with Lini oleum virginale (O) on counts of lactobacilli and coliforms and enzymatic activities in faeces of rats were studied. The rats (n = 60) were divided into 5 groups of 12 subjects. The animals were fed on a high fat diet (10%) for 8 weeks of experiment. Colon cancer was induced by the application of 1,2-dimethylhydrazine (DMH) twice a week in a dose of 20 mg/kg s.c. in groups G2-G5. The rats in group 1 (control 1) received a diet without any supplements. The rats in group 2 (control 2) received 1,2 DMH without any supplements. The rats in group 3 received PRO, group 4 PRO and PRE, and group 5 received PRO and O. A significant decrease (P < 0.05) of coliforms was found out after the application of PRO, PRO-O, and PRO-PRE in comparison with control group G2. Significantly higher (P < 0.05) counts of lactobacilli were determined after the application of PRO-O and PRO-PRE. Significantly lower (P < 0.001) activities of β-galactosidase, β-glucuronidase and α-glucosidase were observed in PRO, PRO-PRE and PRO-O, while in the case of the enzyme β-glucosidase the activity was lower only after the addition of PRO-O. The protective effect of lactobacilli was observed in the order PRO-O, PRO-PRE, and PRO. It was shown that combinations of PRO-O and PRO-PRE had a synergistic effect which was higher than the effect of administering only PRO.

Keywords: L. plantarum; inulin; 1,2-dimethylhydrazine; lactobacilli; coliforms

Published: March 31, 2011  Show citation

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Strojný L, Bomba A, Hijová E, Chmelárová A, Mojžišová G, Bertková I, et al.. Effects of a probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats. Czech J. Anim. Sci. 2011;56(3):99-106. doi: 10.17221/56/2010-CJAS.
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