Czech J. Anim. Sci., 2009, 54(8):359-364 | DOI: 10.17221/1661-CJAS

Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs

Z. Vykoukalová1, A. Knoll1,2, S. Čepica2
1 Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry Brno, Brno, Czech Republic
2 Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic

Perilipin, encoded by the PLIN gene, is a lipid droplet-associated phosphoprotein that functions as a key regulator of triacylglycerol storage and hydrolysis in adipocytes. In this study, structure and variability of the porcine PLIN gene were characterised. PCR fragments encompassing exons 1 to 9 and interspersed introns were sequenced and the obtained sequence was deposited in EMBL/GenBank/DDBJ databases (AM931171). Seven silent polymorphisms and one nonsynonymous polymorphism were detected in the coding sequence. The nonsynonymous polymorphism g.627G>A causing an amino acid substitution p.Val3Ile was found only in Meishan and Meishan × Piétrain cross. Associations were studied between loci g.4119A>G and g.7966T>C, and average daily gain (ADG), backfat thickness (BFT) or lean meat content (LMC) in 166 gilts from two herds. Due to supposed complete linkage disequilibrium between loci g.4119A>G and g.7966T>C only genotype combinations AA-TT, AG-TC and GG-CC were detected. A significant difference (P = 0.0290) between GG-CC and AG-TC genotype combinations for BFT in herd 2 and a suggestive difference (P = 0.0516) between GG-CC and AA-TT genotypes for ADG in herd 1 were detected.

Keywords: pig; perilipin; polymorphism; association analysis

Published: August 31, 2009  Show citation

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Vykoukalová Z, Knoll A, Čepica S. Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs. Czech J. Anim. Sci. 2009;54(8):359-364. doi: 10.17221/1661-CJAS.
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