Czech J. Anim. Sci., 2009, 54(4):182-189 | DOI: 10.17221/1745-CJAS

Effects of indole-3-carbinol on metabolic parameters and on lipogenesis and lipolysis in adipocytes 182

M. Okulicz, I. Hertig, J. Chich³owska
Department of Animal Physiology and Biochemistry, Faculty of Animal Breeding and Biology, University of Life Sciences in Poznañ, Poznañ, Poland

: Indole-3-carbinol (I3C) was found to have possible anticarcinogenic, antioxidant and anti-atherogenic effects on the organism. So far, its influence on metabolic pathways has been unknown. This work was the first attempt to determine the carbohydrate and lipid metabolism changes in vivo after administration of 150 mg/kg b.wt./day I3C to male rats. Additionally, the aim of this trial was to evaluate the direct effect of I3C on basal and hormone-induced lipogenesis and lipolysis in isolated rat adipocytes at concentrations 1, 10, 100 μM in vitro. We can corroborate that adipocytes are susceptible to the direct action of I3C. The incubation of adipocytes with I3C at the three above-mentioned concentrations resulted in its influence on restriction of glucose entry into adipocytes in the basal as well as insulin-stimulated conditions. However, it was observed that I3C at these concentrations strongly intensified basic and epinephrine-stimulated lipolysis. I3C also has a significant influence on metabolism in vivo. Its administration to rats caused a significant increase in the content of triglycerides and a decrease in glycogen in the liver. The considerable augmentation of glucose, triglycerides, cholesterol in high-density lipoprotein and insulin with a concomitant decrease in FFA concentrations was noted in the blood serum. I3C did not alter phospholipids, total, free, esterified cholesterol in the serum and the liver cholesterol. The results obtained in vivo and in vivo indicate that the effect of I3C is adverse for the majority of metabolic parameters which were investigated. The most important finding in this study is the effect of I3C on liver steatosis and that the observed lower lipogenesis at higher lipolysis in fat cells may be involved in the mechanism.

Keywords: indole-3-carbinol; lipid metabolism; carbohydrate metabolism; rat

Published: April 30, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Okulicz M, Hertig I, Chich³owska J. Effects of indole-3-carbinol on metabolic parameters and on lipogenesis and lipolysis in adipocytes 182. Czech J. Anim. Sci. 2009;54(4):182-189. doi: 10.17221/1745-CJAS.
Download citation

References

  1. Bjeldanes L.F., Kim J.Y., Grose K.R., Bartholomew J.C., Bradfield C.A. (1991): Aromatic hydrocarbon responsiveness-receptor agonists generated from indole-3carbinol in vitro and in vivo: Comparisons with 2,3,7,8-tetrachlorodibenzo-p-dioxin. Proceedings of National Academy Sciences USA, 88, 9543-9547. Go to original source... Go to PubMed...
  2. Bradlow H.L., Michnovicz J., Telang N.T., Osborne M.P. (1991): Effects of dietary indole-3-carbinol on estradiol metabolism and spontaneous mammary tumors in mice. Carcinogenesis, 12, 1571-1574. Go to original source... Go to PubMed...
  3. Bradlow H.L., Sepcovic D.W., Telang N.T., Osborne M.P. (1999): Multifunctional aspects of the action of indole3-carbinol as an antitumor agent. Annals of the New York Academy Sciences, 889, 204-213. Go to original source... Go to PubMed...
  4. Brignall M.S. (2001): Prevention and treatment of cancer with indole-3-carbinol. Alternative Medicine Review, 6, 580-589. Go to PubMed...
  5. Coppack S.W., Jensen M.D., Miles J.M. (1994): In vivo regulation of lipolysis in humans. In vivo regulation of lipolysis in humans. Journal of Lipid Research, 35, 177-193. Go to original source... Go to PubMed...
  6. Demacker P.N., Hijmans A.G., Vos-Janssen H.E., van't Laar A., Jansen A.P. (1980): A study of the use of polyethylene glycol in estimating cholesterol in high-density lipoprotein. Clinical Chemistry, 26, 1775-1779. Go to original source...
  7. Duncombe D. (1964): The colorimetric micro-determination of nonesterified fatty acids in plasma. Clinica Chimica Acta, 9, 122-125. Go to original source... Go to PubMed...
  8. Dunn S.E., LeBlanc G.A. (1994): Hypocholesterolemic properties of plant indoles. Inhibition of acyl-CoA:cholesterol acyltransferase activity and reduction of serum LDL/VLDL cholesterol levels by glucobrassicin derivatives. Biochemical Pharmacology, 47, 359-364. Go to original source... Go to PubMed...
  9. Folch J., Lees M., Sloane G.S.H. (1975): A simple method of the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry, 226, 497-509. Go to original source...
  10. Foster L.B., Dunn R.T. (1973): Stable reagents for determination of serum triglycerides by colorimetric Hatzsch condensation method. Clinical Chemistry, 19, 338-340. Go to original source...
  11. Gibbons G.F., Islam K., Pease R. (2000): Mobilisation of triacylglycerol stores. Biochimica et Biophysica Acta, 1483, 37-57. Go to original source... Go to PubMed...
  12. Hugget A.S.G., Nixon D.A. (1957): Use of glucose-oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose. Lancet, 2, 368-370. Go to original source... Go to PubMed...
  13. Kim D.J., Han B.S., Ahn B., Hasegawa R., Shirai T., Ito N., Tsuda H. (1997): Enhancement by indole-3-carbinol of liver and thyroid gland neoplastic development in a rat medium-term multiorgan carcinogenesis model. Carcinogenesis, 18, 377-381. Go to original source... Go to PubMed...
  14. LeBlanc G.A., Stuart J.D., Dunn S.E., Baldwin W.S. (1994): Effect of the plant compound indole-3-carbinol on hepatic cholesterol homoeostasis. Food and Chemical Toxicology, 32, 633-639. Go to original source... Go to PubMed...
  15. Manson M.M., Ball H.W., Barrett M.C., Clark H.L., Judah D.J., Williamson G., Neal G.E. (1997): Mechanism of action of dietary chemoprotective agents in rat liver: induction of phase I and II drug metabolizing enzymes and aflatoxin B1 metabolism. Carcinogenesis, 18, 1729-1738. Go to original source... Go to PubMed...
  16. Nanjee M.N., Verhagen H., van Poppel G., Rompelberg C.J.M., van Bladeren P.J., Miller N.E. (1996): Do dietary phytochemicals with cytochrome P-450 enzyme-inducing activity increase high-density-lipoprotein concentrations in humans? American Journal of Clinical Nutrition, 64, 706-711. Go to original source... Go to PubMed...
  17. Okulicz M., Bialik I., Chich³owska J. (2005): The timedependent effect of gluconasturtiin and phenethyl isothiocyanate on metabolic and antioxidative parameters in rats. Journal of Animal Physiology and Animal Nutrition, 89, 367-372. Go to original source... Go to PubMed...
  18. Park J.Y., Shigenaga M.K., Ames B.N. (1996): Induction of cytochrome P4501A1 by 2,3,7,8-tetrachlorodibenzop-dioxin or indolo(3,2-b)carbazole is associated with oxidative DNA damage. Proceedings of the National Academy of Sciences USA, 93, 2322-2327. Go to original source... Go to PubMed...
  19. Richmond W. (1973): Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clinical Chemistry, 19, 1350-1356. Go to original source...
  20. Rodbell M. (1964): Metabolism of isolated fat cells. The Journal of Biological Chemistry, 239, 375-380. Go to original source... Go to PubMed...
  21. Stresser D.M., Williams D.E., Griffin D.A., Bailey G.S. (1995): Mechanisms of tumor modulation by indole-3carbinol. Disposition and excretion in male Fischer 344 rats. Drug Metabolism and Disposition, 23, 965-975. Go to original source... Go to PubMed...
  22. Szkudelska K., Nogowski L., Szkudelski T. (2000): Genistein affects lipogenesis and lipolysis in isolated rat adipocytes. The Journal of Steroid Biochemistry and Molecular Biology, 75, 265-271. Go to original source... Go to PubMed...
  23. Szkudelska K., Szkudelski T., Nogowski L. (2002): Daidzein, coumestrol and zearalenone affect lipogenesis and lipolysis in rat adipocytes. Phytomedcine, 9, 338-345. Go to original source... Go to PubMed...
  24. Szkudelska K., Nogowski L., Nowicka E., Szkudelski T. (2007): In vivo metabolic effects of naringenin in the ethanol consuming rat and the effect of naringenin on adipocytes in vitro. The Journal of Animal Physiology and Animal Nutrition, 91, 91-99. Go to original source... Go to PubMed...
  25. Szkudelski T., Szkudelska K. (2002): Streptozotocin iduces lipolysis in rat adipocytes in vitro. Physiological Research, 51, 255-259. Go to original source...
  26. Wortelboer H.M., de Kruif C.A., van Iersel A.A.J., Falke H.E., Noordhoek J., Blaauboer B.J. (1992): Acid reaction products of indole-3-carbinol and their effects on cytochrome P450 and phase II enzymes in rat and monkey hepatocytes. Biochemical Pharmacology, 43, 1439-1447. Go to original source... Go to PubMed...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.