Czech J. Anim. Sci., 2009, 54(2):84-91 | DOI: 10.17221/1665-CJAS

The use of bacterial inoculants for grass silage: their effects on nutrient composition and fermentation parameters in grass silages

D. Jalè1, A. Lauková1, M. Simonová1, Z. Váradyová1, P. Homolka2
1 Institute of Animal Physiology, Slovak Academy of Sciences, Koıice, Slovak Republic
2 Research Institute of Animal Production, Prague-Uhĝínìves, Czech Republic

The effect of three microbial inoculants (Lactobacillus plantarum CCM 4000, L. fermentum LF2, and Enterococcus faecium CCM 4231) on the fermentation and nutritive value of orchard grass silage was studied under laboratory conditions. The first-cut orchard grass (280 g of dry matter/kg) was ensiled at 21°C for 105 days. All inoculants were applied at 1.0 × 10(9) CFU/ml. Uninoculated silage served as control. After inoculation, the chopped orchard grass was ensiled in 40 (1 l) plastic jars divided into four groups. The counts of the silage inoculants dominated on day 21 of ensiling: CCM 4231 strain amounted to 9.40 ± 0.30 (log10) CFU/g followed by LF2 (8.69 ± 0.39 CFU/g) and by CCM 4000 (7.55 ± 0.39 CFU/g). However, on day 105 (the end of ensiling) the highest counts of L. plantarum CCM 4000 were determined. Overall, microbial inoculants generally had a positive effect on orchard grass silage characteristics in terms of lower pH and higher lactic acid concentration. The inoculants significantly increased the lactic to acetic acid ratio in inoculated silages. The total concentration of acids (acetic, propionic, n-butyric, lactic acid) was 2-3 times higher in inoculated silages compared to control silage. The percentage proportion of fatty acids - SFA, UFA, SCFA and MCFA - was similar in all grass silages. Only the proportions of LCFA - α- linolenic acid (C18:3) were lower (P < 0.001) while those of oleic acid (C18:1) and linoleic acid (C18:2) were higher (P < 0.001) in inoculated silages in comparison with control silage.

Keywords: orchard grass; silage; bacterial inoculants; quality

Published: February 28, 2009  Show citation

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Jalè D, Lauková A, Simonová M, Váradyová Z, Homolka P. The use of bacterial inoculants for grass silage: their effects on nutrient composition and fermentation parameters in grass silages. Czech J. Anim. Sci. 2009;54(2):84-91. doi: 10.17221/1665-CJAS.
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    References

    1. AOAC (1990): Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists. Arlington, VA, USA, 1230 pp.
    2. Bessa R.J., Santos-Silva J., Ribeiro J.M., Portugal A.V. (2000): Reticulo-rumen biohydrogenation and enrichment of ruminal edible products with linoleic acid conjugated isomers. Applied Environmental Microbiology, 63, 201-211. Go to original source...
    3. Cherney D.R.J., Alessi M.A., Cherney J.H. (2006): Influence of grass species and sample preparation on ensiling characteristics. Crop Science, 46, 256-263. Go to original source...
    4. Chilliard Y., Ferlay A., Doreau M. (2001): Effect of different type of forages, animal fat or marine oils in cow's diet on milk fat secretion and composition, especially conjugated linoleic acid (CLA) and polyunsaturated fatty acids. Livestock Production Science, 70, 31-48. Go to original source...
    5. Coakley M., Ross R.P., Nordgren M., Fitzgerald G., Devery R., Stanton C. (2003): Conjugated linoleic acid biosynthesis by human-derived Bifidobacterium species. Journal of Applied Microbiology, 94, 138-145. Go to original source... Go to PubMed...
    6. Dewhurst R.J, King P.J. (1998): Effects of extended wilting, shading and chemical additives on the fatty acids in laboratory grass silages. Grass and Forage Science, 50, 249-258. Go to original source...
    7. Dewhurst D.J., Scollan N.D., Lee M.R.F., Ougham H.J., Humpreys M.O. (2003): Forage breeding and management to increase the beneficial fatty acid content of ruminal products. Proceedings of Nutrition Society, 62, 329-336. Go to original source... Go to PubMed...
    8. Doreau M., Poncet C. (2000): Ruminal biohydrogenation of fatty acids originating from fresh and preserved grass. Reproduction, Nutrition and Development, 40, 201-209.
    9. Driehuis F., Oude Elferink S.J.W.H., Van Wikselaar P.G. (2001): Fermentation characteristics and aerobic stability of grass silage inoculated with Lactobacillus buchneri with or without homofermentative lactic acid bacteria. Grass and Forage Science, 56, 330-343. Go to original source...
    10. Elgersma A., Tamminga S., Ellen G. (2006): Modifying milk composition through forage. Animal Feed Science and Technology, 131, 207-225. Go to original source...
    11. Filya I., Muck R.E., Contreras-Govea F.E. (2007): Inoculant effects on alfalfa silage: fermentation products and nutritive value. Journal of Dairy Science, 90, 5108-5114. Go to original source... Go to PubMed...
    12. Goering H.K., Van Soest P.J. (1970): Forage Fiber Analyses. Agriculture Handbook No. 379, USDA, Washington, USA, 1-20.
    13. Huhtanen P., Khalili H., Nousiainen J.I., Rinne M., Jaakkola S., Heikkilä T., Nouisiainen J. (2002): Prediction on the relative intake potential of grass silage by dairy cows. Livestock Production Science, 73, 111-130. Go to original source...
    14. Huhtanen P., Nousiainen J.I., Khalili H., Jaakkola S., Heikkilä T. (2003): Relationship between silage fermentation characteristics and milk production parameters: analyses of literature data. Livestock Production Science, 81, 57-73. Go to original source...
    15. Jenkins T.C. (1993): Lipid metabolism in the rumen. Journal of Dairy Science, 76, 3851-3863. Go to original source... Go to PubMed...
    16. Lauková A., Mareková M., Javorskŭ P. (1993): Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231. Letters of Applied Microbiology, 16, 257-260. Go to original source...
    17. Marciòáková M. (2006): Probiotic microorganisms in the feed and the digestive tract of animals and their role in prevention, [PhD. Thesis.] Koıice, SR, 1-145.
    18. Marciòáková M., Simonová M., Lauková A. (2004): Probiotic properties of Enterococcus faecium EF9296 strain isolated from silage. Acta Veterinaria Brno, 73, 513-519. Go to original source...
    19. Marciòáková M., Lauková A., Simonová M., Strompfová V., Koréneková B., Naï P. (2008): Probiotic properties of Enterococcus faecium EF9296 strain isolated from silage. Czech Journal of Animal Science, 53, 336-345. Go to original source...
    20. McAllister T.A., Feniuk R., Mir Z., Mir P., Selinger L.B., Cheng K.J. (1998): Inoculants for alfalfa silage: Effect on aerobic stability, digestibility and the growth performance of feedlot steers. Livestock Production Science, 53, 171-181. Go to original source...
    21. Merry R.J., Jones R., Theodorou M.K. (2000): The conservation of grass. In: Hopkins A. (ed.): Grass - its Production and Utilization. 3rd ed., Blackwell Science Ltd., Oxford, UK.
    22. Muck E., Contreras F.E., Mertens D.R. (2007): Silage inoculant effects on in vitro rumen fermentation. Journal of Animal Science, 85, 284 pp.
    23. Nadeau E.M.G., Russell J.R., Buxton D.R. (2000): Intake, digestibility and composition of orchard grass and alfalfa silages treated with cellulase, inoculant, and formic acid fed to lambs. Journal of Animal Science, 78, 2980-2989. Go to original source... Go to PubMed...
    24. Nemcová R. (1989): Rumen lactobacilli and their impact for the practice, [Ph.D. Thesis.] Koıice, SR, 1-170.
    25. Rooke J.A., Maya F.M., Arnold J.A., Armstrong D.G. (1988): The chemical composition and nutritive value of grass silages prepared with no additive or with the application of additives either Lactobacillus plantarum or formic acid. Grass and Forage Science, 43, 87-95. Go to original source...
    26. Saarisalo E., Skyttä E., Jaakkola S. (2004): Effects of wilted grass silages varying in fermentation quality on rumen fermentation of dairy cows. Journal of Animal and Feed Sciences, 13, 199-202. Go to original source...
    27. Saarisalo E., Skyttä E., Haikara A., Jalava T., Jaakkola S. (2007): Screening and selection of lactic acid bacteria strains suitable for ensiling grass. Journal of Applied Microbiology, 102, 327-336. Go to original source... Go to PubMed...
    28. Schroeder G.F., Gagliostro G.A., Bargo F., Delahoy J.E., Muller L.D. (2004): Effects of fat supplementation on milk production and composition of dairy cows on pasture: a review. Livestock Production Science, 86, 1-18. Go to original source...
    29. Shaver R.D. (2003): Practical application of new forage quality tests. In: Proceedings of the 6th Western Dairy Management Conference, Reno, USA, 22-25.
    30. Sieber R., Collomb M., Aeschliemann A., Jelen P., Eyer H. (2004): Impact of microbial cultures on conjugated linoleic acid in dairy products - a review. International Dairy Journal, 14, 1-15. Go to original source...
    31. Sukhija P.S., Palmquist D.L. (1988): Rapid method for the determination of total fatty acid content and composition of feedstuffs and faeces. Journal of Agricultural and Food Chemistry, 36, 1202-1206. Go to original source...
    32. Strompfová V. (2004): Production of bacteriocins and probiotic properties of microorganisms in the animals digestive tract and their physiological impact, [Ph.D. Thesis.] IAP SAS, Koıice, SR, 1-117.
    33. Tyrolová Y., Vŭborná A. (2008): Effect of the stage of maturity on the leaf percentage of lucerne and the effect of additives on silage characteristics. Czech Journal of Animal Science, 53, 330-335. Go to original source...
    34. Váradyová Z., Baran M., Zeleòák I. (2005): Comparison of two in vitro fermentation gas production methods using both rumen fluid and faecal inoculum from sheep. Animal Feed Science and Technology, 123-124, 81-94. Go to original source...

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