Czech J. Anim. Sci., 2007, 52(11):387-393 | DOI: 10.17221/2325-CJAS
Contamination of cow's raw milk by psychrotrophic and mesophilic microflora in relation to selected factors
- Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic
The objective of the paper was to analyse the influence of dairy cow management technology, milking method, predipping and summer grazing on the contamination of cow's raw milk by mesophilic (TBC), psychrotrophic (PBC), lipolytic (PLiBC) and proteolytic (PPrBC) bacteria. The values of TBC, PBC, PLiBC and PPrBC in bulk milk samples were determined by the culture method according to IDF standards. Investigations were carried out in nine stables of seven dairy farms from January 2005 to June 2006. Summer grazing has the most marked influence on the values of studied parameters. Farms with summer grazing had a lower microbial contamination of milk compared to farms without grazing and the difference was statistically highly significant in all studied parameters (P < 0.001). A positive effect of predipping on a reduction in the values of milk microbial contamination was proved while the difference between farms with predipping and those without it was on a significance leve lP < 0.05 to 0.001 except PLiBC. A comparison of the influence of dairy cow management technology indicated the lowest values of all microbiological indicators in loose cubicle littered housing, higher values were determined in stanchion littered housing and the highest in loose slatted-floor housing. A statistical difference between the technologies was proved mainly in TBC (P < 0.001). Farms with milking in milking parlours had a lower microbial contamination of milk compared to farms that used the in-stall milking pipeline system but the difference was statistically significant only in TBC (P < 0.05).
Keywords: milk; total bacterial count; psychrotrophic bacteria count; lipolytic bacteria count; proteolytic bacteria count; management technology; predipping; grazing
Published: November 30, 2007 Show citation
References
- Blowey R.W., Collis K. (1992): Effect of premilking teat disinfection on mastitis incidence, total bacterial count, cell count and milk-yield in 3 dairy herds. Vet. Rec., 130, 175-178.
Go to original source...
Go to PubMed...
- Brouček J., Mihina Š., Ryba Š., Tongeľ P., Kišac P., Uhrinčať M., Hanus A. (2006): Effects of high air temperatures on milk efficiency in dairy cows. Czech J. Anim. Sci., 51, 93-101.
Go to original source...
- Burdová O., Baranová M., Lauková A., Róžańska H., Rola J.G. (2002): Hygiene of pasteurized milk depending on psychrotrophic microorganisms. Bull. Vet. Inst. Pulawy, 46, 325-329.
- Cempírková R. (2002): Psychrotrophic vs. total bacterial counts in bulk milk samples. Vet. Med. - Czech, 47, 227-233.
Go to original source...
- Chen L. Daniel R.M., Coolbear T. (2003): Detection and impact of protease and lipase activities in milk and milk powders. Int. Dairy J., 13, 255-275.
Go to original source...
- Choi I.W., Jeon I.J. (1993): Patterns of fatty acids released from milk fat by residual lipase during storage of ultrahigh temperature processed milk. J. Dairy Sci., 76, 78-85.
Go to original source...
- Goldberg J.J., Wildman E.E., Pankey J.W., Kunkel J.R., Howard D.B., Murphy B.M.(1992): The influence of intensively managed rotational grazing, traditional continuous grazing, and confinement housing on bulk tank milk quality and udder health. J. Dairy Sci., 75, 96-104.
Go to original source...
Go to PubMed...
- Gonzalo C., Carriedo J.A., Beneitez E., Juárez M.T., De La Fuente L.F., Primitivo S.F. (2006): Short communication: Bulk tank total bacterial count in dairy sheep: factors of variation and relationship with somatic cell count. J. Dairy Sci. 89, 549-552.
Go to original source...
Go to PubMed...
- Hanuš O., Frelich J., Vyletělová M., Roubal P., Vorlíček Z., Jedelská R. (2004): Technologically difficult, pathogenic and food risky bacterial contamination of raw milk and other materials from dairy cow herds. Czech J. Anim. Sci., 49, 489-499.
Go to original source...
- Heeschen W., Reichmuth J., Tolle A., Ziedler H. (1969): Preservation of milk samples for bacteriologic and cytologic examinations for inhibitors. Milchwissenschaft, 24, 729-734.
- Holm C., Jepsen J., Larsen M., Jespersen L. (2004): Predominant microflora of downgraded Danish bulk tank milk. J. Dairy Sci., 87, 1151-1157.
Go to original source...
Go to PubMed...
- Ingawa K.H., Adkinson R.W., Gough R.H. (1992): Evaluation of gel teat cleaning and sanitizing compound for premilking hygiene. J. Dairy Sci., 75, 1224-1232.
Go to original source...
Go to PubMed...
- Jayarao B.M., Piplat S.R., Sawant A.A., Wolfgang D.R., Hedge N.V. (2004): Guidelines for monitoring bulk tank milk somatic cell and bacterial counts. J. Dairy Sci., 87, 3561-3573.
Go to original source...
Go to PubMed...
- Matta H., Punj V. (1999): Isolation and identification of lipolytic, psychrotrophic, spore forming bacteria from raw milk. Int. J. Dairy Technol., 52, 59-62.
Go to original source...
- McKinnon C.H., Rowlands J., Bramley A.J. (1990): The effect of udder preparation before milking and contamination from the milking plant on bacterial numbers in bulk milk of eight herds. J. Dairy Res., 57, 307- 318.
Go to original source...
Go to PubMed...
- Regula G., Badertscher R., Schaeren W., Dalla Torre M., Danuser J. (2002): The effect of animal friendly housing systems on milk quality. Milchwissenschaft, 57, 428-431.
- Vyletělová M., Benda P., Hanuš O., Kopunecz P. (1999): Determination of total counts of psychrotrophic bacteria in pool milk samples and their relation to total counts of microorganisms. Czech J. Food Sci., 17, 216-222.
- Vyletělová M., Ficnar J., Hanuš O. (2000a): Effects of lipolytic enzymes Pseudomonas fluorescens on liberation of fatty acids from milk fat. Czech J. Food Sci., 18, 175-182.
Go to original source...
- Vyletělová M., Hanuš O., Urbanová E., Kopunecz P. (2000b): The occurrence and identification of psychrotrophic bacteria with proteolytic and lipolytic activity in bulk milk samples at storage in primary production. Czech J. Anim. Sci., 45, 373-383.
- Wiking L., Frøst M.B., Larsen L.B., Nielsen J.H. (2002): Effects of storage conditions on lipolysis, proteolysis and sensory attributes in high quality raw milk. Milchwissenschaft, 57, 190-194.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.