Czech J. Anim. Sci., 2006, 51(12):535-542 | DOI: 10.17221/3975-CJAS
Determination of the effect of microbial transglutaminase on technological properties of common carp (Cyprinus carpio L.) meat
- 1 Department of Fishery, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic
- 2 Department of Chemistry, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic
- 3 Research Institute of Fish Culture and Hydrobiology, Faculty of Agriculture, University of South Bohemia, Vodňany, Czech Republic
The aim of this study was to evaluate the effect of microbial transglutaminase (TG) on processed meat of common carp (Cyprinus carpio L.). Three levels of microbial transglutaminase (0.5, 1.0 and 1.5%) combined with three levels of NaCl (0, 1 and 2%), added to support the binding reaction, were examined. For the evaluation of quality changes in restructured fish meat, we used the textural property hardness and water holding capacity (WHC). The results confirmed a strong improvement of texture and water holding capacity after the addition of transglutaminase and salt, whereas the addition of 1% TG + 1% NaCl seems to be the best combination from the economic aspect. Another increase in the addition of TG and NaCl to the fish meat did not lead to further improvement of qualitative properties, as it was in the groups with 0% and 1% NaCl.
Keywords: processing; common carp; enzyme; NaCl; transglutaminase
Published: December 31, 2006 Show citation
ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Ando H., Adachi M., Umeda K., Matsura A., Nonaka M., Uchio R., Tanaka H., Motoki M. (1989): Purification and characteristics of a novel transglutaminase derived form microorganisms. Argic. Biol. Chem., 53, 2613- 2617.
Go to original source...
- Ando H., Matsura A., Susumu H. (1992): Manufacture of transglutaminase with Streptomyces. Jpn. Kokai. Tokyo. Koho., JP 04108381.
- Falcone P., Serafini-Frascassini D., Del Duca S. (1993): Comparative studies of transglutaminase activity and substrates in different organs of Helianthus tuberosus. J. Plant. Physiol., 142, 263-273.
Go to original source...
- Folk J.E. (1980): Transglutaminases. Annu. Rev. Biochem., 49, 517-531.
Go to original source...
Go to PubMed...
- Grantham G.J. (1981): Minced fish technology: a review. FAO Fish. Techn. Paper, 216, 1-72.
- Ha C.R., Iuchi I. (1998): Enzyme responsible for egg envelope (Chorion) hardening in fish. Purification and partial characterization of two Transglutaminases associated with their substrate, unfertilized egg chorion of the rainbow trout (Oncorhynchus mykiss). J. Biochem. Tokyo, 124, 917-926.
Go to original source...
Go to PubMed...
- Hall M., Wang R., Van-Antwerpen R., Sottrup-Jensen L., Soederhaell K. (1999): The crayfish plasma clotting protein. A vitellogenin-related protein responsible for clot formation in crustacean blood. Proc. Nat. Acad. Sci., USA, 96, 1965-1970.
Go to original source...
Go to PubMed...
- Kudo S. (1998): Thrombin induces assembly in vitro of viteline envelope components of common carp eggs. J. Exp. Zool., 282, 367-375.
Go to original source...
- Miller F. (1980): The Paoli mechanical deboner. In: Brooker J.R., Martin R.E. (eds.): 3rd National technical Seminar on Mechanical Recovery and Utilization of Fish. Raleigh, USA, 8 (Abstr.).
- Motoki M., Okiyama A., Nonaka M., Tanaka H., Uchio R., Matsura A., Ando H., Umeda K. (1989): Novel transglutaminase manufacture for preparation of protein gelling compounds. Jpn. Kokai. Tokyo. Koho., JP 0127471.
- Nonaka M., Tanaka H., Okiyama A., Motoki M., Ando H., Umeda K., Matsura A. (1989): Polymerization of several proteins by Ca2+ - independent transglutaminase derived from microorganism. Agric. Biol. Chem., 53, 2619-2623.
Go to original source...
- Nozawa H., Mamegoshi S.I., Seki N. (1997): Partial purification and characterization of six transglutaminases from ordinary muscles of various fishes and marine invertebrates. Comp. Biochem. Physiol., B, 118B, 313- 317.
Go to original source...
Go to PubMed...
- Ohtsuka T., Umezawa Y., Nio N., Kubota K. (2001): Comparison of deamidation activity of transglutaminases. J. Food Sci., 66.
Go to original source...
- Pavlíček T. (2001): New methods of processing of fish meat. [Thesis.] Department of Fishery, Faculty of Agriculture, University of South Bohemia, České Budějovice, 130.
- Pipek P. (1995): Technologie masa I. Skriptum, VŠCHT Praha, 145-163.
- Ramírez J., Uresti R., Téllez S., Vázquez M. (2002): Using salt and microbial transglutaminase as binding agents in restructured fish products resembling hams. J. Food Sci.: Food Eng. Phys. Propert., 67, 1778-1784.
Go to original source...
- Rerenstein J.M., Regenstein C.E. (1991): Introduction to Fish Technology. Van Nostrand Reinhold, 269.
- Taylor R. (1972): Beehive. Section 4. Commercially available equipment. In: Martin R.E. (ed.): Proceedings of the Conference on the Mechanical Recovery and Utilization of Fish Meat. Oak Brook, USA, 64.
- Soederhaell K., Evans L.H., Jones J.B. (2000): Review of crustacean immunity. J. Shellfish Res., 19, 647.
- Yeh M.S., Huang C.J., Leu J.H., Lee Y.C., Tsai I.H. (1999): Molecular cloning and characterization of a hemolymph clottable protein from triger shrimp (Penaeus monodon). Eur. J. Biochem., 266, 624-633.
Go to original source...
Go to PubMed...
- Yasueda H., Kumazawa Y., Motoki M. (1994): Purification and characterization of a tissue-type transglutaminase from Red Sea bream (Pagus major). Biosci. Biotech. Biochem., 58, 2041-2045.
Go to original source...
Go to PubMed...
- Zhu Y., Rinzema A., Tramper J., Bol J. (1995): Microbial transglutaminase - a review of its production and application in food processing. Appl. Microbiol. Biotechnol., 44, 277-282.
Go to original source...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.