Czech J. Anim. Sci., 2006, 51(12):503-509 | DOI: 10.17221/3970-CJAS

Evaluation of parentage testing in the Czech population of Holsteincattle

V. Řehout, E. Hradecká, J. Čítek
Agricultural Faculty, University of South Bohemia, České Budějovice, Czech Republic

A set of 233 Holstein calves, their 233 dams and 44 sires from 4 large-sized herds was genotyped for 10 microsatellites recommended by ISAG for paternity/parentage testing. Heterozygosity of microsatellites ranged from 0.607 (SPS115) to 0.835 (TGLA227), and PIC from 0.575 (SPS115) to 0.816 (TGLA227) confirming a high polymorphism of each analysed locus. Their combined exclusion probability reached 0.999, which made them suitable and sufficiently accurate for parentage testing. A conflict between putative parents and calf in at least 2 markers with combined exclusion probability > 0.9 was required to reject parentage. The pedigree was considered incorrect in 25 (10.73%) out of the evaluated progeny/parent trios, of which in 10 samples the genotype of both parents did not match their offspring, and in 2 samples the putative dam was in conflict with the calf genotype. This result shows that the interchange of calves on farms with large-sized herds plays the role as important as the errors in sire identification, or recording mistakes.

Keywords: dairy cattle; parentage testing; heterozygosity; exclusion probability

Published: December 31, 2006  Show citation

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Řehout V, Hradecká E, Čítek J. Evaluation of parentage testing in the Czech population of Holsteincattle. Czech J. Anim. Sci. 2006;51(12):503-509. doi: 10.17221/3970-CJAS.
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