Czech Journal of Animal Science, 2025 (vol. 70), issue 12
Endogenous inhibitory compounds in bovine milk, their significance and methods of detection
Marcela Klimesova, Ludmila Krizova, Hana Nejeschlebova, Oto Hanus, Lenka Vorlova
Czech J. Anim. Sci., 2025, 70(12):499-517 | DOI: 10.17221/146/2025-CJAS 
Raw cow’s milk is one of the most strictly controlled commodities in the food industry. Besides basic hygienic and nutritional parameters, the presence of inhibitory and contaminant substances plays a critical role in technological quality and safety. While the vast majority of inhibitory residues are represented by antibiotics originating from mastitis prevention and therapy, milk also contains a broad spectrum of naturally occurring native antimicrobial compounds. These include immunoglobulins, lactoferrin, lysozyme, lactoperoxidase, phosphatases, lipases, and proteases acting as integral elements of the innate immune system within the...
Effect of extruded flaxseed supplementation during the indoor fattening of yearling bulls on beef carcass, meat composition, and fatty acid profileOriginal Paper
Emir Mujiæ, Javier Mateo, Mahsa Dehnavi, Halil Omanoviæ, Aida D¾aferoviæ, Munevera Begiæ, Selma Èorbo
Czech J. Anim. Sci., 2025, 70(12):518-527 | DOI: 10.17221/5/2025-CJAS 
This study investigates the impact of supplementing extruded flaxseed to the diet of yearling bulls during the indoor fattening on beef carcass quality, meat composition, and fatty acid profile. Twenty male crossbred calves (Holstein × Simmental) were divided into two groups: control group and flaxseed-supplemented group, each with 10 calves. The control group received a conventional diet, while the flaxseed group was fed the same diet enriched with 5% extruded flaxseed on a dry-matter basis. The study revealed no significant differences in growth performance, carcass weight, or yield between the two groups. However, the flaxseed-supplemented...
Impact of puerperal disorders on early culling and milk production in Slovak Spotted dairy cowsOriginal Paper
Karolína Pále¹ová, Jozef Bujko, Nina Moravèíková, Hana Vostrá-Vydrová, Adrián Halvoník, Lubo¹ Vostrý, Radovan Kasarda
Czech J. Anim. Sci., 2025, 70(12):528-537 | DOI: 10.17221/122/2025-CJAS 
Puerperal diseases are major postpartum complications in dairy cattle and may compromise both survival and production performance. This study evaluated how specific puerperal diseases influence early culling risk and standardised 305-day milk traits in Slovak Spotted cows. A total of 792 animals were clinically assessed during early postpartum and classified as healthy or affected by ketosis, metritis, retained foetal membranes, parturient paresis, or by comorbid diseases, defined as the concurrent occurrence of two or more disorders. Logistic regression models indicated that ketosis was associated with the highest odds of culling compared with...
Lactoferrin – The protective component of goat colostrum and milkOriginal Paper
Pavlína Navrátilová, Klára Bartáková, Jan Pospí¹il, Lenka Vorlová, Tomá¹ Kopec, Oto Hanu¹, Hana Nejeschlebová, Eva Samková, Josef Kuèera, Radoslava Jedelská
Czech J. Anim. Sci., 2025, 70(12):538-553 | DOI: 10.17221/123/2025-CJAS 
Lactoferrin (LF) is an important bioactive component of colostrum, is critical for the development of immunity in the newborns, and it is an important component of the mammary gland defence system. LF is also an important biomolecule in terms of promoting and restoring the human health. The aim of our study was to monitor the dynamics of changes in lactoferrin concentration in goat milk at varying stages of lactation and its correlation with selected components and physicochemical parameters. Colostrum (n = 24) and milk (n = 120) samples were obtained from 12 goats by hand milking. Lactoferrin was determined using reversed-phase...
