Czech J. Anim. Sci., 2025, 70(12):518-527 | DOI: 10.17221/5/2025-CJAS

Effect of extruded flaxseed supplementation during the indoor fattening of yearling bulls on beef carcass, meat composition, and fatty acid profileOriginal Paper

Emir Mujić ORCID...1, Javier Mateo2, Mahsa Dehnavi2, Halil Omanović3, Aida Džaferović1, Munevera Begić3, Selma Čorbo3
1 Biotechnical Faculty, University of Bihac, Bihać, Bosnia and Herzegovina
2 Department of Food Hygiene and Technology, Faculty of Veterinary Sciences, University of León, Campus de Vegazana, León, Spain
3 Faculty of Agriculture and Food Sciences, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

This study investigates the impact of supplementing extruded flaxseed to the diet of yearling bulls during the indoor fattening on beef carcass quality, meat composition, and fatty acid profile. Twenty male crossbred calves (Holstein × Simmental) were divided into two groups: control group and flaxseed-supplemented group, each with 10 calves. The control group received a conventional diet, while the flaxseed group was fed the same diet enriched with 5% extruded flaxseed on a dry-matter basis. The study revealed no significant differences in growth performance, carcass weight, or yield between the two groups. However, the flaxseed-supplemented group exhibited a higher intramuscular fat content, which was likely due to the increased energy intake from the flaxseed lipid content. Additionally, flaxseed supplementation improved the fatty acid profile of beef by increasing the percentage of monounsaturated fatty acids (MUFA) and reducing the saturated fatty acid (SFA) percentage and the n-6/n-3 polyunsaturated fatty acid ratio. Despite these improvements, the study did not reveal any significant increase in the proportion of n-3 fatty acids in the meat. These findings suggest that while flaxseed supplementation enhances the nutritional profile of beef, further research is needed to optimise the balance of energy intake to maximise the increase in n-3 fatty acids.

Keywords: extruded flaxseed; Holstein-Simmental crossbreds; indoor fattening; intramuscular fat; monounsaturated fatty acids; n-6, n-3 ratio

Received: January 13, 2025; Accepted: December 11, 2025; Published: December 30, 2025  Show citation

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Mujić E, Mateo J, Dehnavi M, Omanović H, Džaferović A, Begić M, Čorbo S. Effect of extruded flaxseed supplementation during the indoor fattening of yearling bulls on beef carcass, meat composition, and fatty acid profile. Czech J. Anim. Sci. 2025;70(12):518-527. doi: 10.17221/5/2025-CJAS.
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References

  1. Alberti P, Gomez I, Mendizabal JA, Ripoll G, Barahona M, Sarries V, Realini C. Effect of whole linseed and rumen-protected conjugated linoleic acid enriched diets on feedlot performance, carcass characteristics, and adipose tissue development in young Holstein bulls. Meat Sci. 2013 Jun;94(2):208-14. Go to original source... Go to PubMed...
  2. AOAC. Official methods of analysis of AOAC International. 16th ed. Gaithersburg (MD): AOAC International; 1999.
  3. Aldai N, Dugan MER, Rolland DC, Kramer JKG. Survey of the fatty acid composition of Canadian beef: Backfat and longissimus lumborum muscle. Can J Anim Sci. 2009 Sep;89(3):315-29. Go to original source...
  4. Barton L, Marounek M, Kudrna V, Bures D, Zahradkova R. Growth performance and fatty acid profiles of intramuscular and subcutaneous fat from Limousin and Charolais heifers fed extruded linseed. Meat Sci. 2007 Jul;76(3):517-23. Go to original source... Go to PubMed...
  5. Casaburi A, Piombino P, Nychas GJE, Villani F, Ercolini D. Bacterial populations and the volatilome associated to meat spoilage. Food Microbiol. 2015 Feb;45(Pt A):83-102. Go to original source... Go to PubMed...
  6. Davis H, Magistrali A, Butler G, Stergiadis S. Nutritional benefits from fatty acids in cereal-based products from conventional, organic and milk-based farming systems. Foods. 2022 Mar 1;11(5):646. Go to original source... Go to PubMed...
  7. EFSA. Opinion of the Scientific Panel on Dietetic Products, Nutrition and Allergies (NDA) on a request from the Commission relating to the evaluation of allergenic foods for labeling purposes. EFSA J. 2004 Mar 25;32:1-197.
  8. European Union. Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes. Off J Eur Union. 2010 Oct 20;L 276:33-79.
  9. Gebauer SK, Psota TL, Harris WS, Kris-Etherton PM. n-3 fatty acid dietary recommendations and food sources to achieve essentiality and cardiovascular benefits. Am J Clin Nutr. 2006 Jun;83(6_Suppl):1526S-35S. Go to original source... Go to PubMed...
  10. Giacomino S, Lopez-Fandino R, Ferreyra V, Pellegrino N, Fournier M, Apro N, Frias J. Extruded flaxseed meal enhances the nutritional quality of cereal-based products. Plant Foods Hum Nutr. 2013 Jun;68(2):131-6. Go to original source... Go to PubMed...
  11. Gomez I, Beriain MJ, Mendizabal JA, Realini CE, Purroy A. Shelf life of ground beef enriched with omega-3 and/or conjugated linoleic acid and use of grape seed extract to inhibit lipid oxidation. Food Sci Nutr. 2016 Jan;4(1):67-79. Go to original source... Go to PubMed...
  12. Joseph JD, Ackman RG. Capillary column gas chromatographic method for analysis of encapsulated fish oils and fish oil ethyl esters: Collaborative study. J AOAC Int. 1992 May-Jun;75(3):488-506. Go to original source...
  13. Kim CM, Kim JH, Chung TY, Park KK. Effects of flaxseed diets on fattening response of Hanwoo cattle: 1. Performance and carcass characteristics. Asian-Australas J Anim Sci. 2004 Sep;17(9):1241-6. Go to original source...
  14. Kronberg SL, Barcelo-Coblijn G, Shin J, Lee K, Murphy EJ. Bovine muscle n-3 fatty acid content is increased with flaxseed feeding. Lipids. 2006 Nov;41(11):1059-66. Go to original source... Go to PubMed...
  15. Law on Protection and Welfare of Animals. Official Gazette of Bosnia and Herzegovina. 2009;25:1-17.
  16. Lee EJ, Shin HS. Development of a freshness indicator for monitoring the quality of beef during storage. Food Sci Biotechnol. 2019 Dec;28(6):1899-906. Go to original source... Go to PubMed...
  17. Leiva PM, Frutos AE, Lavandera J, Simoncini MS, Labaque MC, Pina CI, Gonzalez MA. Effect of flaxseed and flaxseed oil supplemented in caiman diet on meat fatty acids. Trop Anim Health Prod. 2021 Nov;53(1):1-11. Go to original source... Go to PubMed...
  18. Long S, Liu S, Wu D, Mahfuz S, Piao X. Effects of dietary fatty acids from different sources on growth performance, meat quality, muscle fatty acid deposition, and antioxidant capacity in broilers. Animals (Basel). 2020 Mar 17;10(3):508. Go to original source... Go to PubMed...
  19. Mir NA, Tyagi PK, Biswas AK, Mandal AB, Kumar F, Verma AK. Inclusion of flaxseed, broken rice and DDGS in broiler chicken ration alters the fatty acid profile and oxidative stability. Eur J Lipid Sci Technol. 2018 Jun;120(6):1700470. Go to original source...
  20. Monson F, Sanudo C, Sierra I. Influence of cattle breed and ageing time on textural meat quality. Meat Sci. 2004 Dec;68(4):595-602. Go to original source... Go to PubMed...
  21. Nassu RT, Dugan MER, He ML, McAllister TA, Aalhus JL, Aldai N, Kramer JKG. Effects of feeding flaxseed to beef cows given forage-based diets on fatty acids of longissimus thoracis muscle and backfat. Meat Sci. 2011 Nov;89(3):469-77. Go to original source... Go to PubMed...
  22. Petricevic M, Niksic D, Delic N, Stamenic T, Petricevic V, Samolovac L, Mandic V. The study of the share of tissues in bovine carcass parts under the influence of the flaxseed diet. Biotechnol Anim Husb. 2020;36(2):155-65. Go to original source...
  23. Pouzo L, Fanego N, Santini FJ, Descalzo A, Pavan E. Animal performance, carcass characteristics and beef fatty acid profile of grazing steers supplemented with corn grain and increasing amounts of flaxseed. Livest Sci. 2015 Aug;178:140-9. Go to original source...
  24. Ripoll G, Blanco M, Alberti P, Panea B, Joy M, Casasus I. Effect of breeds and diet on beef quality including consumer preferences. J Sci Food Agric. 2014 Apr;94(5):983-92. Go to original source... Go to PubMed...
  25. Robelin J, Geay Y. Estimation of muscle, fat and bone proportions of carcass and cuts in young beef bulls using the 6th rib. Ann Zootech. 1975;24(3):255-65.
  26. Park PW, Goins RE. In situ preparation of fatty acid methyl esters for analysis of fatty acid composition in foods. J Food Sci. 1994 Nov;59(6):1262-6. Go to original source...
  27. Serra X, Gil M, Gispert M, Guerrero L, Oliver MA, Sanudo C, Campo MM, Panea B, Olleta JL, Quintanilla R, Piedrafita J. Characterisation of young bulls of the Bruna dels Pirineus cattle breed. Meat Sci. 2004 Feb;66(2):425-36. Go to original source... Go to PubMed...
  28. Simopoulos AP. Omega-3 fatty acids in inflammation and autoimmune diseases. J Am Coll Nutr. 2002 Dec;21(6):495-505. Go to original source... Go to PubMed...
  29. Skrivan M, Englmaierova M, Taubner T, Skrivanova E. Effects of dietary hemp seed and flaxseed on growth performance, meat fatty acid compositions, liver tocopherol concentration and bone strength of cockerels. Animals (Basel). 2020 Mar 12;10(3):458. Go to original source... Go to PubMed...
  30. Vahmani P, Mapiye C, Prieto N, Rolland DC, McAllister TA, Aalhus JL, Dugan MER. The scope for manipulating the polyunsaturated fatty acid content of beef: A review. J Anim Sci Biotechnol. 2015 Jul 16;6(1):29. Go to original source... Go to PubMed...
  31. Van Dael P. Role of n-3 long-chain polyunsaturated fatty acids in human nutrition and health: Review of recent studies and recommendations. Nutr Res Pract. 2021 Apr;15(2):137-59. Go to original source... Go to PubMed...
  32. Wood JD, Enser M, Fisher AV, Nute GR, Sheard PR, Richardson RI, Whittington FM. Fat deposition, fatty acid composition and meat quality: A review. Meat Sci. 2008 Apr;78(4):343-58. Go to original source... Go to PubMed...
  33. Xu L, Wei Z, Guo B, Bai R, Liu J, Li Y, Pi Y. Flaxseed meal and its application in animal husbandry: A review. Agriculture. 2022 Nov 28;12(12):2027. Go to original source...

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