Czech J. Anim. Sci., 2026, 71(2):67-78 | DOI: 10.17221/173/2025-CJAS

Effect of dietary administration of Mentha piperita alone and in combination with Artemisia abrotanum on growth performance, body composition, physicochemical properties and amino acid composition of meat in Eimeria-challenged broilersOriginal Paper

David Zapletal ORCID...1, Martina Kosťuková ORCID...1, Lenka Rozsypalová ORCID...1, Vlastimil Šimek ORCID...1, František Ježek ORCID...2, Josef Kameník ORCID...2, Radka Dobšíková ORCID...1
1 Department of Animal Breeding, Animal Nutrition and Biochemistry, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic
2 Department of Animal Origin Food and Gastronomy Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic

This study aimed to evaluate the effects of dietary administration of peppermint (Mentha piperita) alone and in combination with southernwood (Artemisia abrotanum) on performance, body and carcass composition, physicochemical characteristics, and amino acid composition of breast meat in broilers under Eimeria challenge. A total of 160 14-day-old male broilers (Ross 308) were randomly assigned to four dietary groups: the control (C) group was fed a basal diet; the CC group received a grower diet containing the coccidiostat robenidine; the M group was fed the diets with the addition of 20 g peppermint/kg of diet; the MS group was fed the diets with the addition of 10 g peppermint + 10 g of southernwood/kg of diet. The addition of peppermint to the diet increased final live weight compared to the CC group (P < 0.05). The highest value of dressing percentage was found in the M group as compared to the CC group (P < 0.01) and C group (P < 0.05). Compared with the C group, a higher intramuscular fat content was found in the breast meat of broilers in both the M and MS groups (P < 0.01; P < 0.05, resp.). Concerning the colour of meat, lower values for yellowness b* and chroma C* characteristics were observed in the C group compared to the MS group (P < 0.05) and mainly to the M group (P < 0.01). In spite of the lower crude protein content (P < 0.05) in the meat of the MS group, the highest content of all essential amino acids (P < 0.05) as well as the total content of all assessed amino acids (P < 0.01) were found in the meat of the MS group, resulting in a considerable increase in the nutritional value of the breast meat proteins of these broilers.

Keywords: amino acid; breast muscle; carcass trait; coccidiosis; meat nutrition; phytogenic additive

Received: December 9, 2025; Accepted: January 19, 2026; Prepublished online: February 24, 2026; Published: February 26, 2026  Show citation

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Zapletal D, Kosťuková M, Rozsypalová L, Šimek V, Ježek F, Kameník J, Dobšíková R. Effect of dietary administration of Mentha piperita alone and in combination with Artemisia abrotanum on growth performance, body composition, physicochemical properties and amino acid composition of meat in Eimeria-challenged broilers. Czech Journal of Animal Science. 2026;71(2):67-78. doi: 10.17221/173/2025-CJAS.
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References

  1. Abdel-Wareth AA, Lohakare JD. Effect of dietary supplementation of peppermint on performance, egg quality, and serum metabolic profile of Hy-Line Brown hens during the late laying period. Anim Feed Sci Tech. 2014 Nov;197:114-20. Go to original source...
  2. Abdel-Wareth AAA, Kehraus S, Sudekum KH. Peppermint and its respective active component in diets of broiler chickens: Growth performance, viability, economics, meat physicochemical properties, and carcass characteristics. Poult Sci. 2019 Sep 1;98(9):3850-9. Go to original source... Go to PubMed...
  3. Aminzade B, Karami B, Lotfi E. Meat quality characteristics in Japanese quails fed with Mentha piperita plant. Anim Biol Anim Husb. 2012 Jun;4(1):20-3.
  4. Anyaoku IC, Igwilo IO, Ezekwesili CN. Phytomedicinal and nutritional values of Mentha piperita. Int J Res Publ Rev. 2023 Nov;4(11):3029-37.
  5. Asadi N, Husseini SD, Tohidian MT, Abdali N, Mimandipoure A, Rafieian-Kopaei M, Bahmani M. Performance of broilers supplemented with peppermint (Mentha piperita L.) powder. J Evid Based Complementary Altern Med. 2017 Oct;22(4):703-6. Erratum in: J Evid Based Integr Med. 2018 Oct 16;23:2515690X18809744. Go to original source... Go to PubMed...
  6. Bai M, Liu H, Zhang Y, Wang S, Shao Y, Xiong X, Hu X, Yu R, Lan W, Cui Y, Kong X. Peppermint extract improves egg production and quality, increases antioxidant capacity, and alters cecal microbiota in late-phase laying hens. Front Microbiol. 2023 Sep 21;14:1252785. Go to original source... Go to PubMed...
  7. Beigi M, Torki-Harchegani M, Pirbalouti AG. Quantity and chemical composition of essential oil of peppermint (Mentha × piperita L.) leaves under different drying methods. Int J Food Prop. 2018 Apr;21(1):267-76. Go to original source...
  8. Brenes A, Roura E. Essential oils in poultry nutrition: Main effects and modes of action. Anim Feed Sci Technol. 2010 Mar;158(1-2):1-14. Go to original source...
  9. Dilawar MA, Mun HS, Rathnayake D, Yang EJ, Seo YS, Park HS, Yang CJ. Egg Quality parameters, production performance and immunity of laying hens supplemented with plant extracts. Animals (Basel). 2021 Mar 31;11(4):975. Go to original source... Go to PubMed...
  10. Ekiert H, Knut E, Swiatkowska J, Klin P, Rzepiela A, Tomczyk M, Szopa A. Artemisia abrotanum L. (Southern Wormwood) - History, current knowledge on the chemistry, biological activity, traditional use and possible new pharmaceutical and cosmetological applications. Molecules. 2021 Apr 25;26(9):2503. Go to original source... Go to PubMed...
  11. Gal R, Zapletal D, Jakesova P, Strakova E. Proximate chemical composition, amino acids profile and minerals content of meat depending on carcass part, sire genotype and sex of meat rabbits. Animals (Basel). 2022 Jun 14;12(12):1537. Go to original source... Go to PubMed...
  12. Gui J, Lin W, Meng C, Cui Y, Lan W, He J, Azad MAK, Kong X. Network pharmacology and molecular docking reveal the mechanism of Chinese herb ultrafine powder improving meat nutritional value in aged laying hens. Poult Sci. 2025 May;104(5):105047. Go to original source... Go to PubMed...
  13. He CQ, Liu Y, Dai Y, Yao YL, Li MC, Xie J, Wang YB, Qu XY, Deng YY. The influence of plant essential oil/palygorskite composite on growth performance, blood parameters and intestinal morphology of broiler chickens. Ital J Anim Sci. 2023 Oct;22(1):1073-82. Go to original source...
  14. Honikel KO. Reference methods for the assessment of physical characteristics of meat. Meat Sci. 1998 Aug;49(4):447-57. Go to original source... Go to PubMed...
  15. Hussein SM, M'Sadeq SA, Beski SSM, Mahmood AL, Frankel TL. Different combinations of peppermint, chamomile and a yeast prebiotic have different impacts on production and severity of intestinal and bursal abnormalities of broilers challenged with coccidiosis. Ital J Anim Sci. 2021 Oct;20:1924-34. Go to original source...
  16. Khempaka S, Pudpila U, Molee W. Effect of dried peppermint (Mentha cordifolia) on growth performance, nutrient digestibility, carcass traits, antioxidant properties, and ammonia production in broilers. J Appl Poultry Res. 2013 Dec;22(4):904-12. Go to original source...
  17. Lin ZN, Ye L, Li ZW, Huang XS, Lu Z, Yang YQ, Xing HW, Bai JY, Ying ZY. Chinese herb feed additives improved the growth performance, meat quality, and nutrient digestibility parameters of pigs. Animal Model Exp Med. 2020 Mar 16;3(1):47-54. Go to original source... Go to PubMed...
  18. Muthamilselvan T, Kuo TF, Wu YC, Yang WC. Herbal remedies for Coccidiosis control: A review of plants, compounds, and anticoccidial actions. Evid Based Complement Alternat Med. 2016;2016:2657981. Go to original source... Go to PubMed...
  19. Obianwuna UE, Chang X, Oleforuh-Okoleh VU, Onu PN, Zhang H, Qiu K, Wu S. Phytobiotics in poultry: Revolutionizing broiler chicken nutrition with plant-derived gut health enhancers. J Anim Sci Biotechnol. 2024 Dec 9;15(1):169. Go to original source... Go to PubMed...
  20. Ocak N, Erener G, Ak F, Sungu M, Altop A, Ozmen A. Performance of broilers fed diets supplemented with dry peppermint (Mentha piperita L.) or thyme (Thymus vulgaris L.) leaves as growth promoter source. Czech J Anim Sci. 2008 Apr;53(4):169-75. Go to original source...
  21. Petricevic V, Doskovic V, Lukic M, Skrbic Z, Rakonjac S, Petricevic M, Stanojkovic A. Effect of peppermint (Mentha piperita L.) in broiler chicken diet on production parameters, slaughter characteristics and gut microbial composition. Large Anim Rev. 2021 Apr;27(2):103-7.
  22. Ravi S, Biswas A, Kolluri G, Pipaliya G, Deo C, Rokade J, Goyari K, Parte P. Effect of dietary supplementation of herbal immuno-modulator on growth performance, immune response and serum biochemical indices of broiler chickens. Sci Rep. 2025 Nov 13;15(1):39771. Go to original source... Go to PubMed...
  23. Reda FM, Kamal M, Mahmoud HK, Rudayni HA, Allam AA, Khafaga AF, Khan MMH, Mohamed RS. Effect of a mixture of peppermint and clove oils on growth performance, carcass traits, blood parameters, and gut microbiota in broiler chickens. Poult Sci. 2025 Nov;104(11):105718. Go to original source... Go to PubMed...
  24. Strakova E, Vsetickova L, Suchy P, Kutlvasr M. Effect of dehulled lupin seeds in feed mixture on muscle protein quality of ducklings. Czech J Anim Sci. 2025 Feb;70(2):64-71. Go to original source...
  25. Tumova E, Teimouri A. Fat deposition in the broiler chicken: A review. Sci Agric Bohem. 2010;41(2):121-8.
  26. Tyl J, Tumova E, Chodova D. Effects of two protein levels on the performance of chicken males with different growth intensities. Czech J Anim Sci. 2025 May;70(5):194-201. Go to original source...
  27. Vitula F, Suchy P, Strakova E, Karaskova K, Zapletal D, Kroupa L. Energy value of meat in selected species of feathered game. Acta Vet Brno. 2011;80(2):197-202. Go to original source...
  28. Wang Q, Wang L, Li L, Sun M, Li P, Yu Y, Zhang Y, Xu Z, Gao P, Ma J, Liu X. Effects of dietary supplementation of fermented Artemisia argyi on growth performance, slaughter performance, and meat quality in broilers. Poult Sci. 2024 Apr;103(4):103545. Go to original source... Go to PubMed...
  29. Yu M, Jeon JO, Cho HM, Hong JS, Kim YB, Nawarathne SR, Wickramasuriya SS, Yi YJ, Lee H, Wan V, Ng NKJ, Tan CH, Heo JM. Broiler responses to dietary 3,4,5-trihydroxybenzoic acid and oregano extracts under Eimeria challenge conditions. J Anim Sci Technol. 2021 Nov;63(6):1362-75. Go to original source... Go to PubMed...
  30. Zapletal D, Jakesova P, Zakova E, Simek V, Strakova E. Growth performance, mortality and body and carcass characteristics of rabbit fatteners related to crossbreeding of Mecklenburger Schecke sires with dam line of HYLA rabbits. Czech J Anim Sci. 2020 Sep;65(9):337-45. Go to original source...
  31. Zapletal D, Dobsikova R, Simek V, Kamenik J, Jezek F. Growth performance, carcass composition, physico-chemical traits and amino acid profile of meat depending on wormwood (Artemisia absinthium L.) dietary supplementation in broilers. Arch Anim Breed. 2024 Jan 5;67(1):1-12. Go to original source... Go to PubMed...
  32. Zapletal D, Dobsikova R, Kostukova M, Simek V, Rozsypalova L, Kamenik J, Jezek F. Effect of wormwood (Artemisia absinthium L.) supplementation to diet on performance, body composition, immune organs, gut morphology, amino acid composition and sensory attributes of breast meat in Eimeria-challenged chickens. Ital J Anim Sci. 2025a Apr;24:1015-27. Go to original source...
  33. Zapletal D, Dobsikova R, Kostukova M, Simek V, Stribrna H, Koudela B. Broilers responses to dietary wormwood administration under Eimeria-challenged conditions. Czech J Anim Sci. 2025b Feb;70(2):55-63. Go to original source...

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