Stachurska Anna author Brodacki Antoni author Liss Marta author text journal article 2018 The objective of this study was to estimate the frequencies of alleles which produce coat colour in Polish Coldblood horse population, and to verify the hypothesis that coat colour is not considered in its selection. The analysis included 35 928 horses and their parents having been registered in the studbook over a half-century. Allele frequencies in Agouti (A), Extension (E), Dun (D), Roan (Rn), and Grey (G) loci, in parental and offspring generations, were estimated according to test matings and the square root of recessive phenotype frequency. The population structure is in Hardy-Weinberg equilibrium only at E locus and coat colour is regarded by breeders. Black horses are favoured. Higher E locus homozygosity in blacks than in bays makes it easier to obtain black foals. Dun-diluted, roan and grey coat colours are undesirable and the population has come to consist almost uniformly of basic coat colours. These results show the importance of studies on population genetic structure, which despite no formal criteria for breeding for colour, can considerably change through generations. generation; population genetic structure; test mating 10.17221/107/2018-CJAS https://cjas.agriculturejournals.cz/artkey/cjs-201811-0005.php https://cjas.agriculturejournals.cz/artkey/cjs-201811-0005.php continuing 63 11 462 472 2018 12121819 periodical academic journal