RT Journal Article
SR Electronic
A1 Slanina, Tomáš
A1 Miškeje, Michal
A1 Tirpák, Filip
A1 Błaszczyk, Martyna
A1 Stawarz, Robert
A1 Massányi, Peter
T1 Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility
JF Czech Journal of Animal Science
YR 2018
VO 63
IS 4
SP 127
OP 135
DO 10.17221/79/2017-CJAS
UL https://cjas.agriculturejournals.cz/artkey/cjs-201804-0001.php
AB The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine - from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision<sup>®</sup> and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87-9.90%) if compared to 5°C (2.12-4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.