RT Journal Article
SR Electronic
A1 Zhan, X.A.
A1 Wang, H.F.
A1 Yuan, D.
A1 Wang, Y.X.
A1 Zhu, F.
T1 Comparison of different forms of dietary selenium supplementation on gene expression of cytoplasmic thioredoxin reductase, selenoprotein P, and selenoprotein W in broilers
JF Czech Journal of Animal Science
YR 2014
VO 59
IS 12
SP 571
OP 578
DO 10.17221/7797-CJAS
UL https://cjas.agriculturejournals.cz/artkey/cjs-201412-0006.php
AB Effects of different forms of dietary selenium (Se) supplementation on gene expression of cytoplasmic thioredoxin reductase (TrxR1), selenoprotein P (SelP), and selenoprotein W (SelW) in broilers were investigated. A total of six hundred Ross 308 broilers (1-day-old) with similar body weight were randomly divided into three groups, each of which included 5 replicates of 40 birds. These three treatments received the same basal diet with only background Se level of 0.04 mg Se/kg, supplemented with 0.15 mg Se/kg as sodium selenite (SS) or l-selenomethionine (l-Se-Met) or d-selenomethionine (d-Se-Met) for 42 days. The SS supplemented diet increased TrxR1 activity in liver (P < 0.01) and kidney (P < 0.01) as well as SelP concentration in serum (P < 0.05) and liver (P < 0.01) more than the d-Se-Met supplemented diet. The addition of SS also highly increased liver (P < 0.01) and kidney (P < 0.01) TrxR1 activities of broilers in comparison with broilers fed l-Se-Met diet. In addition, liver TrxR1 activity in l-Se-Met group was higher than that in d-Se-Met group (P < 0.05). Liver and kidney mRNA levels of TrxR1 and SelP as well as breast muscle SelW mRNA level were significantly increased by l- and d-Se-Met supplementation in comparison with SS supplementation (P < 0.01), while the d-Se-Met group showed more effective (P < 0.01) than the l-Se-Met group in increasing the mRNA levels of TrxR1 and SelP in liver and kidney. Therefore, dietary l-Se-Met and d-Se-Met supplementation could improve mRNA levels of different selenoproteins studied and reduce amounts of TrxR1 and SelP in broilers compared with SS. Besides, l-Se-Met is more effective than d-Se-Met in raising TrxR1 activity and decreasing mRNA abundance of TrxR1 and SelP in broilers.