RT Journal Article
SR Electronic
A1 Simon, M.
A1 Antalíková, J.
A1 Horovská, Ľ.
A1 Jankovičová, J.
A1 Fábryová, K.
A1 Hluchý, S.
A1 Chrenek, P.
A1 Tančin, V.
T1 Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens
JF Czech Journal of Animal Science
YR 2009
VO 54
IS 6
SP 270
OP 276
DO 10.17221/1728-CJAS
UL https://cjas.agriculturejournals.cz/artkey/cjs-200906-0004.php
AB Studies that involved testing monoclonal antibodies (mAbs) for cross-species reactivity proved to be efficient for the identification of previously unrecognized antigens in a number of different species. Twenty-six mAbs specific to different bovine CD (cluster defined) antigens (CD9, CD18, CD45R, CD41/61, CD62L, MHC class I and bovine IgG light chain molecule) were assayed for reactivity with rabbit peripheral blood leukocytes. Four of the mAbs recognizing CD9 and CD41/61 were reactive with rabbit platelets or granulocytes. These were investigated further by immunoblotting and immunohistochemical staining. The study identified CD9 and CD41/61 molecules on rabbit cells by mAbs IVA-50 and IVA-38. It showed that IVA-50 is a new valuable CD9 reagent for rabbit immunology which could be used for immunofluorescence staining or ELISA assay, immunohistological and molecular studies of rabbit CD9 antigen. IVA-38 recognizes the CD41/61 on rabbit platelets in indirect immunofluorescence and ELISA assay.