Simon M. author Antalíková J. author Horovská Ľ. author Jankovičová J. author Fábryová K. author Hluchý S. author Chrenek P. author Tančin V. author text journal article 2009 Studies that involved testing monoclonal antibodies (mAbs) for cross-species reactivity proved to be efficient for the identification of previously unrecognized antigens in a number of different species. Twenty-six mAbs specific to different bovine CD (cluster defined) antigens (CD9, CD18, CD45R, CD41/61, CD62L, MHC class I and bovine IgG light chain molecule) were assayed for reactivity with rabbit peripheral blood leukocytes. Four of the mAbs recognizing CD9 and CD41/61 were reactive with rabbit platelets or granulocytes. These were investigated further by immunoblotting and immunohistochemical staining. The study identified CD9 and CD41/61 molecules on rabbit cells by mAbs IVA-50 and IVA-38. It showed that IVA-50 is a new valuable CD9 reagent for rabbit immunology which could be used for immunofluorescence staining or ELISA assay, immunohistological and molecular studies of rabbit CD9 antigen. IVA-38 recognizes the CD41/61 on rabbit platelets in indirect immunofluorescence and ELISA assay. blood cells; tissue; CD9; CD41/61 10.17221/1728-CJAS https://cjas.agriculturejournals.cz/artkey/cjs-200906-0004.php https://cjas.agriculturejournals.cz/artkey/cjs-200906-0004.php continuing 54 6 270 276 2009 12121819 periodical academic journal